The effect of hippocampal NR2B-containing NMDA receptors on chronic cognitive dysfunction in rats with temporal lobe epilepsy.

IF 1.2 Q4 CLINICAL NEUROLOGY

Acta Epileptologica Pub Date : 2023-01-03 DOI:10.1186/s42494-022-00111-2

Xiaoqing Luo, Cheng Li, Xiaoli Yu, Guangtao Kuang, Xiaolu Wang, Jufang Liang, Jun Jiang

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Abstract

Background: We have previously reported that hippocampal long-term potentiation (LTP) was suppressed in temporal lobe epilepsy (TLE) rats. The N-methyl-D-aspartic acid receptors containing 2B subunit (NR2B-NMDARs) are indispensable to induce the LTP of hippocampus. However, it is still unknown whether the NR2B-NMDARs are implied with the cognitive dysfunction in TLE rats.

Methods: The TLE model was lithium chloride-pilocarpine (li-pilo) model. Morris water maze test was used to evaluate the cognitive function of epileptic rats. Expression of hippocampal NR2B-NMDAs was evaluated by western blotting. Stereotactic injection of NMDA, an agonist of NR2B-NMDARs, into the hippocampus of TLE rats was used to investigate the role of NR2B-NMDARs on cognitive dysfunction.

Results: Cognitive function TLE rats was significantly reduced compared with controls in the Morris water maze test (P < 0.05). Western blotting data showed the down-expression of hippocampal NR2B-NMDARs and p-NR2B in TLE rats compared with the control (P < 0.05). In addition, hippocampal stereolocalization of NMDA injection improved partially the learning and memory in TLE rats (P < 0.05).

Conclusions: Down-expression and low activity of hippocampal NR2B-NMDARs may be implied with chronic cognitive dysfunction in TLE rats.

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含有NMDA受体的海马NR2B对颞叶癫痫大鼠慢性认知功能障碍的影响

背景：我们之前报道过颞叶癫痫（TLE）大鼠海马长期增强（LTP）被抑制。含有2B亚基的n -甲基- d -天冬氨酸受体（NR2B-NMDARs）是诱导海马LTP不可缺少的。然而，NR2B-NMDARs是否与TLE大鼠认知功能障碍有关尚不清楚。方法：采用氯化锂-匹罗卡品（li-pilo）模型。采用Morris水迷宫试验评价癫痫大鼠的认知功能。western blot检测海马nr2b - nmda的表达。采用立体定向注射NR2B-NMDARs激动剂NMDA进入TLE大鼠海马，研究NR2B-NMDARs对认知功能障碍的作用。结果：与对照组相比，Morris水迷宫实验显著降低了TLE大鼠的认知功能(P)。结论：海马NR2B-NMDARs的低表达和低活性可能与TLE大鼠的慢性认知功能障碍有关。

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