ANTIOXIDANT ACTIVITY AND PHENOLIC PROFILE OF FRUITS FROM SOME ROMANIAN SHRUB FRUIT VARIETIES

Q4 Agricultural and Biological Sciences
C. Popa, S. Avramescu, E. Oprea, C. Nicola, M. Paraschiv, R. Hertzog, Mihail Coman
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引用次数: 0

Abstract

The berries are rich in polyphenols, vitamins, and other bioactive compounds, and exhibit antioxidant, antimicrobial, anti-inflammatory and anticancer activities. In this work, antioxidant activity (AA) and a phenolic profile of berries fruits belonging to the Aronia melanocarpa (Michx.) Elliott, ʹMelromʹ cv., Lonicera caerulea var. kamtschatica, ʹKamiʹ cv., and Sambucus nigra L., ʹElromʹ cv. were studied. All three cvs. were previously patented by the Research Institute for Fruit Growing Pitesti, Romania. Alcoholic extracts from berries were obtained by four methods: continuous, maceration, ultrasounds and microwaves-assisted. The antioxidant content of extracts was evaluated by an HPLC method. AA was determined by DPPH free radical scavenging method. Total phenol, flavonoid, and anthocyanin contents were determined by spectrophotometric methods. The AA values were reported as equivalents (µg mL-1 extract) caffeic and gallic acids, morin, quercetin, rutin, and are in concordance with the results of the chromatographic method. In addition, the chromatographic method allowed identifying the p-coumaric acid, caffeic acid, chlorogenic acid, rutin, and epicatechin. The experimental results have shown that analyzed fruit extracts have high antioxidant activity, due mainly to flavonoids and anthocyanins in high concentration. Therefore, extracts from mentioned berries can be used in different nutraceutical products with high antioxidant potential.
罗马尼亚灌木品种果实的抗氧化活性和酚类成分
浆果富含多酚、维生素和其他生物活性化合物,具有抗氧化、抗菌、抗炎和抗癌活性。在这项工作中,研究了黑果Aronia blackcarpa(Michx.)Elliott,Melrom,cv.,Lonicera caerulea var.kamtschatica,Kami,cv.和Sambucus nigra L.,Elrom的浆果果实的抗氧化活性(AA)和酚类特征。所有三个cv。之前由罗马尼亚Pitesti水果种植研究所获得专利。采用连续、浸渍、超声波和微波辅助四种方法从浆果中提取乙醇提取物。采用高效液相色谱法对提取物中抗氧化剂的含量进行了评价。采用DPPH自由基清除法测定AA。采用分光光度法测定了总酚、黄酮和花青素的含量。AA值报告为咖啡酸和没食子酸、桑色素、槲皮素、芦丁的当量(µg mL-1提取物),与色谱法的结果一致。此外,色谱法还可以鉴定对香豆酸、咖啡酸、绿原酸、芦丁和表儿茶素。实验结果表明,所分析的果实提取物具有较高的抗氧化活性,主要是由于高浓度的黄酮类化合物和花青素。因此,上述浆果的提取物可用于不同的具有高抗氧化潜力的营养品中。
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来源期刊
Fruit Growing Research
Fruit Growing Research Agricultural and Biological Sciences-Agronomy and Crop Science
CiteScore
0.10
自引率
0.00%
发文量
0
审稿时长
8 weeks
期刊介绍: FRUIT GROWING RESEARCH is an international journal, which has been issued under the care of the Research Institute for Fruit Growing Pitesti, Romania. Fruit growing research is an annual journal aimed at disseminating significant research and original papers on genetic resources, breeding of deciduous fruit trees species; fruit sustainable technologies – environment protection; propagation, virology and tissue culture, postharvest, that can be of interest to a wide audience of plant scientists in all areas of fruit growing. An international Editorial Board advises the journal.
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