Panicum miliaceum L. and Bone Healing Properties in Rat Model of Femur Fracture by Activating Phosphate Stimulating Macrophages via BMP2 and RANK Signaling Pathway

IF 0.6 4区医学 Q4 CHEMISTRY, MEDICINAL

Pharmacognosy Magazine Pub Date : 2023-09-06 DOI:10.1177/09731296231188459

Yubing Zhang, Qiu Wang, Hansong Pan, Hongye Xu, Wei Deng, Xiaohai Sun

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Abstract

The aim of this study was to investigate the effect of Panicum miliaceum involved in the repair of femur bone fracture in rats. In this study, 40 Wistar rats in five groups were studied as healthy, rats with lesion induced by surgery in femur (FX), FX rats treated with P. miliaceum extract (FX +100 and 200 P. miliaceum) and FX rats treated with Osteocare syrup (FX + OC). After 40 days, the serum levels of blood calcium (Ca), phosphorus (P), and alkaline phosphatase (ALP) along with activity of glutathione peroxidase (GPx), superoxide dismutase (SOD), and catalase (CAT) enzymes, and nitric oxide (NO) level were measured. The serum level of cytokines (IL-6, C-reactive protein [CRP], IL-10, IL-1β, and tumor necrosis factor-α [TNF-α]) was also measured by ELISA. The expression of OPG, RANK, RANKL, and BMP-2 genes and proteins were evaluated by real-time PCR and Western blotting techniques, respectively. The results showed that P. miliaceum was able to decrease the activity of ALP enzyme in addition to increasing serum Ca and P levels and GPx, SOD, and CAT activity. P. miliaceum reduces serum IL-6, IL-1β, and TNF-α, and NO levels. Also, the extract of this plant could increase the expression of OPG/RANK/BMP-2 pathway genes and proteins. P. miliaceum can be used as a prodrug in traditional medicine to repair bone fractures and osteoporosis.

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通过BMP2和RANK信号通路激活磷酸刺激巨噬细胞对大鼠股骨骨折模型骨愈合的影响

本研究旨在探讨粟粒对大鼠股骨骨折修复的影响。在本研究中，将40只Wistar大鼠分为五组，分别为健康大鼠、股骨手术损伤大鼠（FX）、用粟粒菌提取物（FX+100和200粟粒菌）治疗的FX大鼠和用Osteocare糖浆（FX+OC）治疗的FX大鼠。40天后，测量血清钙（Ca）、磷（P）和碱性磷酸酶（ALP）水平，以及谷胱甘肽过氧化物酶（GPx）、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性和一氧化氮（NO）水平。还通过ELISA测定了血清细胞因子（IL-6、C-反应蛋白[CRP]、IL-10、IL-1β和肿瘤坏死因子-α[TNF-α]）的水平。分别通过实时PCR和蛋白质印迹技术评估OPG、RANK、RANKL和BMP-2基因和蛋白质的表达。结果表明，粟粒假单胞菌除能提高血清Ca、P水平和GPx、SOD、CAT活性外，还能降低ALP酶活性。米利aceum降低血清IL-6、IL-1β、TNF-α和NO水平。此外，该植物的提取物可以增加OPG/RANK/BMP-2通路基因和蛋白质的表达。米利aceum可作为传统医学中的前药，用于修复骨折和骨质疏松症。

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