PREVALENCE, ANTIMICROBIAL RESISTANCE PROFILES OF CLINICAL AND SUBCLINICAL MASTITIS IN LACTATING COW WITH ASSESSMENT OF TREATMENT TRIAL

IF 0.3 4区农林科学 Q4 VETERINARY SCIENCES

Slovenian Veterinary Research Pub Date : 2023-02-26 DOI:10.26873/svr-1618-2022

Wafaa M El-Neshwy, Abo-Zaid, A.A.,, El-Mekawey, M.F, Soliman, A.H

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引用次数: 0

Abstract

This work investigated the prevalence of bovine mastitis in a dairy farm, Ismalia governrate, Egypt with phynotypic and genotypic clarification of the causative bacteria and their antimicrobial susceptibility. Also a treatment trial with a combination of Cafalexin, Kanamycin was evaluated. The total prevalence of mastitis was 31.82% (119/374) at cow level and 17.01% (247/1452) at quarter level. 261 isolates were detected. (74.33%) belong to staphylococcus spp and (25.67%) to streptococcus spp. as major microorganisms (CNS (42.53%) s.aurus (31.80%) s. uberis (12.26%), s. agalactia (8.81%) and s. dysagalactia(4.59%). The isolates were tested against 15 antimicrobial agents and the highest percentage of resistant bacteria was for AMC, P, C and E. while the lowest rate was for CN, CIP, CL and K. Molecular Characterization of isolated pathogens and antimicrobial resistance genes was performed by PCR on 15 isolates. blaTEM-1 Was the most frequently detected gen followed by aadA1, dfrA1, cmlA, sul1, and tetA. 110 infected udder quarters were enrolled for 21 days to evaluate the treatment with Terrexine LC intramammary suspension 10g on six occasions at 12 h intervals and gentamycin intramuscular injection (1cm/20kg Bw for 3-5 days in cows with systemic reaction. A high significant reduction was recorded for the log10 SSC, log10 TBC and the level of LDH in milk after treatment compared their level before treatment (P-value <0.0001***). The milk season for cows, degree of mastitis or type of microorganism isolated before treatment have no effect on the recovery rate P-value ˃0.05. In conclusion, the emergence of multidrug-resistant strains is greatly increased so antibiotic usage must be Restricted. PCR can help in the rapid detection of the resistant strains. Treatment of mastitis with combination of antimicrobial may reduce drug resistance. Bovine mastitis still needs updated knowledge on the causative microbes and their antibiotic resistance patterns for optimal control and treatment.

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泌乳奶牛临床和亚临床乳腺炎的患病率、耐药性及治疗试验评价

这项工作调查了埃及伊斯马利亚政府奶牛场的奶牛乳腺炎患病率，并对致病细菌及其耐药性进行了表型和基因型分析。此外，还评估了卡法乐新和卡那霉素的联合治疗试验。乳腺炎的总患病率在奶牛水平为31.82%（119/374），在季度水平为17.01%（247/1452）。检出261株分离株。葡萄球菌属（74.33%），链球菌属（25.67%），为主要微生物（CNS（42.53%），金黄色葡萄球菌（31.80%），uberis葡萄球菌（12.26%），无乳链球菌（8.81%），无乳酸链球菌（4.59%）。用聚合酶链式反应对15株分离的病原菌和抗微生物基因进行了分子鉴定。blaTEM-1是最常见的检测基因，其次是aadA1、dfrA1、cmlA、sul1和tetA。110个受感染的乳房被纳入21天的研究，以评估Terrexine LC肌内混悬液10g 6次、间隔12小时和庆大霉素肌内注射的治疗效果（1 cm/20kg Bw，在有全身反应的奶牛中持续3-5天。与治疗前相比，治疗后牛奶中log10 SSC、log10 TBC和LDH水平显著降低（P值<0.00001***），乳腺炎的程度或治疗前分离的微生物类型对恢复率P值没有影响。0.05。总之，耐多药菌株的出现大大增加，因此必须限制抗生素的使用。PCR有助于快速检测耐药菌株。乳腺炎的联合抗菌药物治疗可以减少耐药性。牛乳腺炎仍然需要更新致病微生物及其抗生素耐药性模式的知识，以进行最佳控制和治疗。

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来源期刊

Slovenian Veterinary Research VETERINARY SCIENCES-

CiteScore

0.60

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： SLOVENIAN VETERINARY RESEARCH (ISSN 1580-4003) publishes original articles, which report the results of original research in most areas of biomedicine. The journal also publishes review articles dealing with rapidly developing areas of biomedicine or which update understanding of classical fields of biomedicine, as well as case reports, shorter scientific contributions, letters to the editor, etc.; which have not been published or are under consideration for publication elsewhere. Only papers written in English can be considered.