The functional effects of microRNA-502-5p on colon cancer DLD-1 cells

中华实验外科杂志 Pub Date : 2019-12-08 DOI:10.3760/CMA.J.ISSN.1001-9030.2019.12.025

Lu Liu, W. Lai, He-yang Xu, Yujie Zeng, Qiusheng Lan, Tao Zhang

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Abstract

Objective To investigate the effect of microRNA (miRNA, miR)-502-5p on colon cancer DLD-1 cells. Methods Colon cancer cell line DLD-1 was transfected by miR-502-5p mimic, control mimic, miR-502-5p inhibitor and control inhibitor respectively. The effect of miR-502-5p on the proliferation of DLD-1 cells was detected by cell counting kit-8 (CCK-8) assay. The effect of miR-502-5p on the colony forming ability of DLD-1 cells was detected by plate-cloning assay. Transwell assay was used to evaluate the effect of miR-502-5p on the migration and invasion of colon cells. Results The expression of miR-502-5p was significantly increased (319.31±33.16 vs. 1.08±0.22) in DLD-1 cells after transfection with miR-502-5p mimic (P 0.05), while migration [(621±90) cells vs. (174±32) cells] and invasion [(531±45) cells vs. (132±34) cells] ability were significantly increased (P 0.05), but the migration [(133±50) cells vs. (543±84) cells] and invasion [(119±45) cells vs. (458±57) cells] ability were decreased significantly (P<0.05). Conclusion MiR-502-5p may promote the migration and invasion of DLD-1 cells, but has no effect on the proliferation function. Key words: Colon cancer; MicroRNA-502-5p; Proliferation; Migration; Invasion

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微小RNA-502-5p对癌症DLD-1细胞功能的影响

目的研究微小RNA（miRNA，miR）-502-5p对结肠癌细胞系DLD-1的影响。方法分别用miR-502-5p模拟物、对照模拟物、miR-502-5p-抑制剂和对照抑制剂转染癌症细胞系DLD-1。通过细胞计数试剂盒-8（CCK-8）检测miR-502-5p对DLD-1细胞增殖的影响。通过平板克隆法检测miR-502-5p对DLD-1细胞集落形成能力的影响。Transwell分析用于评估miR-502-5p对结肠细胞迁移和侵袭的影响。结果miR-502-5p模拟物转染DLD-1细胞后，其表达显著增加（319.31±33.16 vs.1.08±0.22）（P 0.05），迁移[（621±90）细胞vs.（174±32）细胞]和侵袭[（531±45）细胞vs.（132±34）细胞]能力显著增强（P 0.05，但迁移能力[（133±50）个细胞对（543±84）个细胞]和侵袭能力[（119±45）个细胞相对（458±57）个细胞）明显下降（P＜0.05）。关键词：结肠癌癌症；MicroRNA-502-5p；扩散；移民；入侵

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