Enhanced insect and fungal resistance of maize callus transgenically expressing a maize E2F regulatory gene

Q1 Agricultural and Biological Sciences

Agri Gene Pub Date : 2019-06-01 DOI:10.1016/j.aggene.2019.100086

Patrick F. Dowd, Eric T. Johnson

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引用次数: 6

Abstract

A maize gene coding for an E2F regulatory protein located in a quantitative trait locus (QTL) for Fusarium ear rot resistance was cloned and transgenically introduced into maize callus. Several of the transformants were more resistant to feeding by corn earworms and fall armyworms, and retarded growth of Fusarium proliferatum compared to GUS control transformants. More effective transformants contained higher levels of E2F product than GUS controls as indicated by antibody detection. Increased and decreased levels of regulated proteins were noted in E2F overexpressing compared to GUS expressing control transformants. These differentially produced proteins were previously reported to be interactors with the E2F protein, suggesting E2F is affecting the production of these proteins. Other proteins coded for by genes reported to interact with the relevant E2F protein and associated phenotypic effects that could be promoting resistance include those that would increase cell resistance to water stress, increase the presence of reactive oxygen species, and increase the density of indigestible components. Although the full complex of responsible proteins regulated by the E2F examined that promote resistance to insects and fungi remains to be determined, overproduction of the E2F in transgenic callus enhances resistance to some maize insect and fungal pests.

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转基因表达玉米E2F调控基因增强玉米愈伤组织抗虫、抗真菌能力

克隆了玉米抗穗腐病数量性状位点E2F调控蛋白基因，并将其导入到玉米愈伤组织中。与GUS对照转化子相比，部分转化子对玉米耳虫和秋粘虫的取食具有较强的抗性，并能延缓增殖镰刀菌的生长。抗体检测表明，更有效的转化子比GUS对照组含有更高水平的E2F产物。与表达GUS的对照转化子相比，E2F过表达的受调控蛋白水平升高或降低。这些差异产生的蛋白质先前被报道为与E2F蛋白相互作用，表明E2F影响这些蛋白质的产生。据报道，基因编码的其他蛋白质与相关的E2F蛋白相互作用，以及相关的表型效应，可能会促进抗性，包括那些会增加细胞对水分胁迫的抵抗力，增加活性氧的存在，增加不可消化成分的密度。虽然E2F调控的促进对昆虫和真菌抗性的完整蛋白复合物仍有待确定，但转基因愈伤组织中E2F的过量产生增强了对某些玉米害虫和真菌害虫的抗性。

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来源期刊

Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.