Detection of Helicobacter pylori genes (CagA and VacA) in municipal drinking water

IF 1.3 Q4 ENVIRONMENTAL SCIENCES
F. Gholami-Borujeni, H. Rahimi, Seyed Mehdi Sajjadi
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引用次数: 1

Abstract

Background: Helicobacter pylori is classified as a carcinogen, and it is also the most common cause of chronic bacterial infection and peptic ulcers. Approximately 45% of people are infected with the bacterium. Methods: In this study, the H. pylori genes, CagA and VacA, were investigated in drinking water, using 100 samples (50 samples from the municipal water supply and 50 samples from the effluent of household water treatment devices). DNA was extracted from colonies with a positive heterotrophic plate count (HPC) for use in molecular testing and microbial identification. The polymerase chain reaction (PCR) was used to identify H. pylori. Results: The study showed that 24% of urban water samples (12% above the World Health Organization [WHO] standards for safe drinking water) and 18% of home water treatment-device samples (4% above the WHO standards) were HPC-positive. The H. pylori genes, CagA and VacA, were identified in 2% of the samples from household water treatment devices and 8% of the municipal water supply samples. Conclusion: The study findings show that H. pylori may be transmitted in drinking water. However, there is currently no strong evidence that the bacteria can survive after the disinfection process in the water supply system. Therefore, the health risks of this bacterium in drinking water are still unknown.
城市饮用水中幽门螺杆菌基因(CagA和VacA)的检测
背景:幽门螺杆菌被列为致癌物质,也是慢性细菌感染和消化性溃疡最常见的原因。大约45%的人感染了这种细菌。方法:在本研究中,使用100个样本(50个来自城市供水的样本和50个来自家庭水处理装置出水的样本)对饮用水中的幽门螺杆菌基因CagA和VacA进行了调查。从具有阳性异养平板计数(HPC)的菌落中提取DNA,用于分子测试和微生物鉴定。采用聚合酶链式反应(PCR)对幽门螺杆菌进行了鉴定。结果:研究表明,24%的城市水样(高于世界卫生组织安全饮用水标准的12%)和18%的家庭水处理设备样品(高于世界卫生组织标准的4%)为HPC-阳性。幽门螺杆菌基因CagA和VacA在2%的家庭水处理设备样本和8%的城市供水样本中被鉴定。结论:研究结果表明幽门螺杆菌可能在饮用水中传播。然而,目前还没有强有力的证据表明,在供水系统中消毒后,细菌可以存活。因此,这种细菌在饮用水中的健康风险仍然未知。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
2.40
自引率
37.50%
发文量
17
审稿时长
12 weeks
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