Effects of p38 MAPK signal pathway inhibition on upper lip scar hyperplasia at different time in rabbits

中华医学美学美容杂志 Pub Date : 2019-06-15 DOI:10.3760/CMA.J.ISSN.1671-0290.2019.03.020

Kai-Ning Jia, Yaoxian Xu, Guo-Rong Yu, J. Yue, Feng Liu

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引用次数: 0

Abstract

Objective To investigate the effect of p38MAPK gene silencing recombinant adenovirus on the expression of target gene in different time and to detect the effect of p38MAPK signal pathway on the upper lip scar hyperplasia at different time to determine the optimal scar treatment time. Methods The adenovirus vector was injected into the scar tissue in 0 week, 1 week and 2 week after cheiloplasty in rabbit. The specimens were harvested in 3 week postoperatively. Four methods including Sirius red staining, immunohistochemical staining (IHC), Western blotting (WB), real-time PC (RT-PCR) were used to quantitatively and quantitatively detect the relative expression levels of p38MAPK and scar-related factors (colⅠ, colⅢ, MMP1, TIMP1). Results Sirius red staining and immunohistochemical staining showed that in 1st week the expression of colⅢ and MMP1 in scar tissue was significantly higher than that in 0 week and 2 week after operation and the expression of colⅠ and TIMP1 was significantly less than that in 0 week and 2 week after operation. The results of WB and RT-PCR were consistent with that of IHC. Conclusions After injection into the upper lip scar tissue with adenovirus in 1 week, the degree of scar hyperplasia is the least. Key words: Rabbits; Cleft lip; Cicatrix, hypertrophic; p38MAPK signaling pathway; Gene silencing; Adenovirus vector

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p38 MAPK信号通路抑制对兔不同时间上唇瘢痕增生的影响

目的研究p38MAPK基因沉默重组腺病毒在不同时间对靶基因表达的影响，并检测p38MAPK信号通路在不同时间上唇瘢痕增生中的作用，以确定最佳瘢痕治疗时间。方法将腺病毒载体分别于兔唇成形术后0周、1周和2周注入瘢痕组织。术后3周采集标本。采用天狼星红染色、免疫组织化学染色（IHC）、蛋白质印迹（WB）、实时PC（RT-PCR）四种方法定量检测p38MAPK和瘢痕相关因子（colⅠ、colⅢ、MMP1、TIMP1）的相对表达水平。结果天狼星红染色和免疫组化染色显示，术后1周瘢痕组织中colⅢ和MMP1的表达明显高于术后0周和2周，colⅠ和TIMP1的表达明显低于术后0周和2周。WB和RT-PCR结果与IHC结果一致。结论腺病毒注入上唇瘢痕组织1周后，瘢痕增生程度最低。关键词：兔子；唇裂；瘢痕，肥大；p38MAPK信号通路；基因沉默；腺病毒载体

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来源期刊

中华医学美学美容杂志

自引率

0.00%

发文量

4641

期刊介绍： "Chinese Journal of Medical Aesthetics and Cosmetology" is a high-end academic journal focusing on the basic theoretical research and clinical application of medical aesthetics and cosmetology. In March 2002, it was included in the statistical source journals of Chinese scientific and technological papers of the Ministry of Science and Technology, and has been included in the full-text retrieval system of "China Journal Network", "Chinese Academic Journals (CD-ROM Edition)" and "China Academic Journals Comprehensive Evaluation Database". Publishes research and applications in cosmetic surgery, cosmetic dermatology, cosmetic dentistry, cosmetic internal medicine, physical cosmetology, drug cosmetology, traditional Chinese medicine cosmetology and beauty care. Columns include: clinical treatises, experimental research, medical aesthetics, experience summaries, case reports, technological innovations, reviews, lectures, etc.