Capric and lauric acid mixture decreased rumen methane production, while combination with nitrate had no further benefit in methane reduction

IF 1.8 4区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
M. Joch, Mariana Vadroňová, M. Češpiva, P. Zabloudilová, A. Výborná, Y. Tyrolová, V. Kudrna, Denisa Tichá, V. Plachý, Z. Hroncova
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Abstract

Abstract This study aimed to evaluate the methane-reducing potential of individual and combined treatments of low levels of nitrate (NIT) and a mixture of capric/lauric acid (CL) in dairy cows. Both in vitro and in vivo experiments were conducted. In the in vitro experiment, the anti-methanogenic effects of NIT (1.825 mmol/l) and CL (250 mg/l; capric acid, 125 mg/l + lauric acid, 125 mg/l) were evaluated in a 2 × 2 factorial design using consecutive batch incubations with rumen fluid. The NIT and CL reduced (P<0.05) methane production by 9.2% and by 21.3%, respectively. However, combining NIT with CL did not show (P>0.05) any benefit in methane reduction compared to the use of CL alone. In the in vivo experiment, eight multiparous dry Holstein cows were fed two diets in a crossover design for two 21-day periods (14 days of adaptation and 7 days of sampling). The treatments were: 1) silage-based basal diet + 100 g stearic acid per cow/d (CON) and 2) silage-based basal diet + 50 g capric acid + 50 g lauric acid per cow/d (CL). Gas emissions were measured using open-circuit respiration chambers. Methane production (g/d) was reduced (by 11.5%; P = 0.012) when the diet was supplemented with CL. However, supplementation with CL increased ruminal ammonia-N concentration (by 28.5%; P = 0.015) and gas ammonia production (g/d; by 37.2%; P = 0.005). Ruminal pH, protozoa count, and total and individual volatile fatty acid concentrations (VFA ) did not differ (P>0.05) between the treatments. Treatment did not affect the intake and apparent total tract digestibility (P>0.05). In conclusion, our results suggest that low CL levels have anti-methanogenic potential. However, low levels of CL may compromise nitrogen use efficiency.
癸酸和月桂酸混合物降低了瘤胃甲烷产量,而与硝酸盐的组合对甲烷的减少没有进一步的促进作用
摘要本研究旨在评估低水平硝酸盐(NIT)和癸酸/月桂酸(CL)混合物单独和联合处理奶牛的甲烷还原潜力。进行了体外和体内实验。在体外实验中,NIT(1.825mmol/l)和CL(250mg/l;癸酸,125mg/l+月桂酸,125g/l)的抗甲烷作用在2×2析因设计中进行了评估,使用瘤胃液连续分批孵育。与单独使用CL相比,NIT和CL降低了甲烷减少的任何益处(P0.05)。在体内实验中,8头多胎干荷斯坦奶牛在两个21天的时期(14天的适应期和7天的采样期)以交叉设计的方式饲喂两种日粮。处理为:1)基于青贮饲料的基础日粮+100克硬脂酸/头牛/d(CON)和2)基于青贮料的基础日料+50克癸酸+50克月桂酸/头牛-d(CL)。使用开路呼吸室测量气体排放。当日粮中添加CL时,甲烷产量(g/d)降低(11.5%;P=0.012)。然而,添加CL增加了瘤胃氨态氮浓度(28.5%;P=0.015)和气氨产量(g/d;37.2%;P=0.005)。瘤胃pH、原生动物计数以及总挥发性脂肪酸和单个挥发性脂肪酸浓度(VFA)在处理之间没有差异(P>0.05)。处理不影响采食量和表观总消化率(P>0.05)。总之,我们的结果表明,低CL水平具有抗甲烷的潜力。然而,低水平的CL可能会损害氮的使用效率。
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来源期刊
Annals of Animal Science
Annals of Animal Science 农林科学-奶制品与动物科学
CiteScore
4.00
自引率
5.30%
发文量
138
审稿时长
6-12 weeks
期刊介绍: Annals of Animal Science accepts original papers and reviews from the different topics of animal science: genetic and farm animal breeding, the biology, physiology and reproduction of animals, animal nutrition and feedstuffs, environment, hygiene and animal production technology, quality of animal origin products, economics and the organization of animal production.
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