Evaluation of genetic fidelity of in vitro-propagated blackberry plants using RAPD and SRAP molecular markers

IF 1 Q3 HORTICULTURE

Horticultural Science Pub Date : 2020-03-31 DOI:10.17221/20/2019-hortsci

O. Borsai, M. Hârța, K. Szabo, C. Kelemen, Flavia Andreea Andrecan, M. Codrea, D. Clapa

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引用次数: 14

Abstract

The aim of this research was to evaluate the genetic uniformity of blackberry plants (Rubus fruticosus L. ‘Loch Ness’ and ‘Chester Thornless’) obtained by micropropagation. Genetic uniformity was analysed by using RAPD and SRAP markers. For the in vitro multiplication, the slightly modified Murashige and Skoog (1962) basal medium was used, supplemented with 0.5 mg/l 6-Benzyladenine (BA), prepared with tap water and 50 g/l wheat starch as the gelling agent. This culture medium ensured the regeneration of well-developed plantlets, with multiplication rates of more than 42 for both cultivars. The in vitro multiplication was carried out in 30 months including 12 subcultures. The plants obtained from the 3rd and 11th subcultures were compared with the mother plants using 64 SRAP primer combinations (eight forward and eight reverse primers) and 20 RAPD primers to check their genetic fidelity. The amplification products were monomorphic in the micropropagated plants and the mother plant. No polymorphism was detected, thus proving the genetic fidelity and uniformity of the micropropagated plants.

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用RAPD和SRAP分子标记评价黑莓离体繁殖植株的遗传保真度

本研究的目的是评价黑莓植株(Rubus fruticosus L. ' Loch Ness '和' Chester Thornless ')的遗传均匀性。利用RAPD和SRAP标记分析遗传均匀性。体外增殖采用稍作修饰的Murashige和Skoog(1962)基础培养基，添加0.5 mg/l 6-苄基ladenine (BA)，以自来水和50 g/l小麦淀粉为胶凝剂制备。该培养基确保了发育良好的植株再生，两个品种的增殖率均超过42%。体外增殖30个月，包括12次传代培养。利用64个SRAP引物组合(8个正向引物和8个反向引物)和20个RAPD引物组合与母株进行遗传保真度比较。扩增产物在微繁植株和母株中呈单态。未检测到多态性，从而证明了微繁殖植株的遗传保真度和均匀性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Horticultural Science Horticulture-园艺

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal publishes results of basic and applied research from all areas of horticulture, fruit-growing, vegetable-growing, wine-making and viticulture, floriculture, ornamental gardening, garden and landscape architecture, concerning plants that are grown under the conditions of European temperate zone, or field plants that are considered as horticultural cultures. Original scientific papers, short communications and review articles are published in the journal. Papers are published in English (British spelling).