Molecular characterization of temple tree rust caused by Coleosporium plumeriae Pat.

Q4 Biochemistry, Genetics and Molecular Biology

Journal of Applied Horticulture Pub Date : 2020-12-25 DOI:10.37855/JAH.2020.V22I03.40

R. Munilakshmi, B. A. Reddy

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Abstract

Temple tree (Plumeria rubra) is a cosmopolitan ornamental flowering and an avenue tree. Generally, Plumeria sp. is affected by several diseases, among them rust caused by Coleosporium plumeriae was found infecting the temple trees in Bengaluru, Karnataka, India with the severity of 80-100 per cent in the early June till end of August 2016-17. Initially, numerous orange coloured rusty pustules were seen on abaxial leaf and these pustules covered the entire leaf causing inward rolling of leaves, necrosis, senescence and finally leaf abscission. Microscopic observation of the rust pustules revealed the presence of golden pale yellow coloured uredospores that varied from globose to ellipsoidal in shape, ranging from 20-40 x 16-28 mm in size, borne on erumpent uredinia and no other spore stages were observed. Molecular identification of this pathogen (Indian isolate) through ITS rDNA region amplification, sequencing and phylogenetic analysis revealed 99 % sequence homology with the China isolate (Accession no. KF879087.1). ITS rDNA region partial sequence of the pathogen was deposited in NCBI, GenBank with accession no. MH656772.

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鸡毛球孢子菌(Coleosporium plumeriae Pat)引起的寺庙树锈病的分子特征。

庙树(Plumeria rubra)是一种世界性的观赏花卉和林荫道树木。一般来说，鸡毛锈病受几种疾病的影响，其中由鸡毛锈病引起的锈病被发现感染了印度卡纳塔克邦班加罗尔的寺庙树木，在2016-17年6月初至8月底，严重程度为80- 100%。最初，叶片背面可见大量桔黄色锈斑脓疱，这些脓疱覆盖整个叶片，导致叶片向内滚动，坏死，衰老，最终脱落。显微镜下可见金黄色的淡黄色尿素孢子，其形状从球形到椭圆形不等，大小为20-40 mm × 16-28 mm，产自毒脲，未见其他孢子期。通过ITS rDNA区扩增、测序和系统发育分析对该病原菌(印度分离株)进行分子鉴定，结果显示该病原菌与中国分离株的同源性达99%。KF879087.1)。将病原菌ITS rDNA区域部分序列存入NCBI, GenBank，登录号:MH656772。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Applied Horticulture Agricultural and Biological Sciences-Horticulture

CiteScore

0.90

自引率

0.00%

发文量

期刊介绍： The Journal of Applied Horticulture (JAH) is an official publication of the Society for the Advancement of Horticulture, founded in 1999. JAH is a triannual publication, publishes papers of original work (or results), & rapid communications and reviews on all aspects of Horticultural Science which can contribute to fundamental and applied research on horticultural plants and their related products. The essential contents of manuscripts must not have been published in other refereed publications. Submission of a manuscript to the Journal implies no concurrent submission elsewhere.