Ameliorative role of melatonin against cypermethrin or gamma irradiation induced testicular damage in male rats

Q4 Health Professions

Iranian Journal of Radiation Research Pub Date : 2020-10-10 DOI:10.52547/IJRR.18.4.765

E. M Kamal El-Dein, Lobna M. Anees

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引用次数: 2

Abstract

Background: This work aimed at studying the protective effect of melatonin (MLT) against cypermethrin or γ-radiation induced testicular damage. Material and Methods: Thirty six Male rats were randomly divided into 6 groups. Control group, group treated with MLT (10 mg/Kg b. wt.), group administered cypermethrin (25 mg/ kg. b. wt.), group 4 was exposed to fractionated whole body γ-radiation (the sum=8 Gy), group 5 was coadministered with MLT (10 mg/Kg .b. wt.) and cypermethrin (25 mg/kg b. wt.) and finally group 6 rats were supplemented with MLT (10 mg/Kg .b. wt.) and subsequently administered fractionated γ-radiation(the sum=8 Gy). After treatment for 29 successive days, all animals groups were investigated for hormonal assay, sperm quality, oxidative indices and histological examination of testicular tissues. Results: Rat’s treatment with -radiation or Cypermethrin showed a significant decrease in body weight, testis, epididymis, ventral prostate and seminal-vesicles weights. Also, a significant increase was observed in the levels of TBARS and H2O2, while a significant reduction occurred in the activity of SOD, CAT and GSH content in testis tissues, in addition to a significant decrease in testosterone hormone. On the other hand, a significant increase occurred in FSH and prolactin hormones .moreover, a significant decrease in sperm count and motility occured beside a significant increase in abnormal forms. Pretreatment of rats with MLT improved the disturbances induced in the tested parameters of rats either received cypermethrin or γ-irradiation. Conclusion: According to the present findings, the ameliorative role of MLT is obvious against cypermethrin or γ-irradiation induced testicular damage probably, due to its antioxidative properties.

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褪黑素对氯氰菊酯或γ射线所致雄性大鼠睾丸损伤的改善作用

背景:研究褪黑素(MLT)对氯氰菊酯或γ辐射致睾丸损伤的保护作用。材料与方法:36只雄性大鼠随机分为6组。对照组、MLT组(10 mg/Kg b. wt.)、氯氰菊酯组(25 mg/Kg)。b. wt.)，第4组接受分次全身γ-辐射(和=8 Gy)，第5组与MLT (10 mg/Kg .b.)联合给药。最后，6组大鼠在对照组基础上添加MLT (10 mg/kg .b)和氯氰菊酯(25 mg/kg .b)。wt.)，随后给予分次γ辐射(和=8 Gy)。连续治疗29 d后，对各组动物进行激素测定、精子质量、氧化指标及睾丸组织组织学检查。结果:-辐射或氯氰菊酯处理大鼠体重、睾丸、附睾、前列腺腹侧及精囊重量均显著降低。睾丸组织中TBARS和H2O2水平显著升高，SOD、CAT和GSH含量显著降低，睾丸激素显著降低。另一方面，卵泡刺激素和催乳素激素显著增加。此外，精子数量和活力显著减少，异常形式显著增加。MLT预处理大鼠对氯氰菊酯和γ辐照大鼠的实验参数均有改善作用。结论:MLT对氯氰菊酯或γ辐照致睾丸损伤有明显的改善作用，可能与其抗氧化作用有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Iranian Journal of Radiation Research RADIOLOGY, NUCLEAR MEDICINE & MEDICAL IMAGING-

CiteScore

0.67

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Iranian Journal of Radiation Research (IJRR) publishes original scientific research and clinical investigations related to radiation oncology, radiation biology, and Medical and health physics. The clinical studies submitted for publication include experimental studies of combined modality treatment, especially chemoradiotherapy approaches, and relevant innovations in hyperthermia, brachytherapy, high LET irradiation, nuclear medicine, dosimetry, tumor imaging, radiation treatment planning, radiosensitizers, and radioprotectors. All manuscripts must pass stringent peer-review and only papers that are rated of high scientific quality are accepted.