Further data on the distribution of Dirofilaria spp. in the Czech Republic in dogs.

IF 1.5 4区医学 Q3 PARASITOLOGY

Folia Parasitologica Pub Date : 2022-03-31 DOI:10.14411/fp.2022.007

J. Juránková, Barbora Šenkyříková Mitková, Marcela Novotná, L. Hofmannová, B. Červená, D. Bowman, D. Modrý

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引用次数: 1

Abstract

Based on previously published data, the Czech Republic is regarded an endemic country of the onchocercid nematodes Dirofilaria immitis (Leidy, 1856) and Dirofilaria repens Railliet et Henry, 1911. Nevertheless, while cases of D. repens are commonly reported from dogs in South Moravia, no recent records of D. immitis are available. Therefore, the present study was performed to clarify the occurrence of both species of Dirofilaria Railliet et Henry, 1910. Blood samples of 551 dogs sampled during 2015 and 2016 were analysed microscopically for presence of microfilariae and blood sera were examined by IDEXX SNAP® 4Dx® test (IDEXX, USA). DNA from blood of microscopically positive dogs was extracted and PCR protocol amplifying fragment of cytochrome c oxidase I (COI) gene was performed; PCR products were then sequenced. All dogs from the Bohemian part of the Czech Republic were negative. The prevalence of D. repens in the Moravian region was 5.7 % (27/476). BLAST analyses of obtained sequences confirmed the presence of D. repens (99-100% identical to KX265049). All sampled animals showed a negative result for D. immitis antigen in IDEXX SNAP® 4Dx® test. Our study confirmed the previously reported occurrence of D. repens in South Moravia and revealed its spreading from the epicentre to the north and west. PCR with subsequent sequencing together with negative results for D. immitis antigen in IDEXX SNAP® 4Dx® test revealed only D. repens infection. A previously published autochthonous infection of dogs with D. immitis in South Moravia was not confirmed.

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关于Dirofilaria spp.在捷克共和国狗中分布的进一步数据。

根据先前公布的数据，捷克共和国被认为是盘尾丝虫病的流行国家(Leidy, 1856)和repens Dirofilaria Railliet et Henry, 1911)。然而，虽然在南摩拉维亚经常报告狗感染repens的病例，但没有最近的immitis记录。因此，本研究旨在阐明两种Dirofilaria Railliet et Henry, 1910的发生情况。对2015年和2016年采集的551只狗的血样进行显微分析，检测微丝蚴的存在，并采用IDEXX SNAP®4Dx®检测(IDEXX, USA)检测血清。从镜检阳性犬血液中提取DNA，进行细胞色素c氧化酶I (COI)基因PCR扩增;然后对PCR产物进行测序。所有来自捷克共和国波西米亚地区的狗都是阴性的。在摩拉维亚地区流行率为5.7%(27/476)。BLAST分析获得的序列证实了D. repens的存在(与KX265049相同99-100%)。所有动物在IDEXX SNAP®4Dx®检测中均显示D. imimtis抗原阴性。我们的研究证实了先前报道的在南摩拉维亚发生的D. repens，并揭示了其从震中向北部和西部蔓延。随后的PCR测序以及IDEXX SNAP®4Dx®检测中D. immitis抗原阴性结果显示只有D. repens感染。先前在南摩拉维亚发表的犬类原生感染阿米特弓形虫病未得到证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Folia Parasitologica 医学-寄生虫学

CiteScore

2.70

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： FOLIA PARASITOLOGICA, issued in online versions, is an international journal that covers the whole field of general, systematic, ecological and experimental parasitology. It publishes original research papers, research notes and review articles. Contributions from all branches of animal parasitology, such as morphology, taxonomy, biology, biochemistry, physiology, immunology, molecular biology and evolution of parasites, and host-parasite relationships, are eligible. Novelty and importance in the international (not local or regional) context are required. New geographical records of parasites, records of new hosts, regional parasite and/or host surveys (if they constitute the principal substance of manuscript), local/regional prevalence surveys of diseases, local/regional studies on epidemiology of well known diseases and of parasite impact on human/animal health, case reports, routine clinical studies and testing of established diagnostic or treatment procedures, will not be considered. One species description will also not be considered unless they include more general information, such as new diagnostic characters, host-parasite associations, phylogenetic implications, etc. Manuscripts found suitable on submission will be reviewed by at least two reviewers.