An integrative approach reveals high species diversity in the primitively segmented spider genus Songthela (Mesothelae: Liphistiidae) from Hunan, China

IF 1.8 2区生物学 Q3 EVOLUTIONARY BIOLOGY

Invertebrate Systematics Pub Date : 2022-02-22 DOI:10.1071/IS21058

Deng-Xu Li, Zhaoyang Chen, Fengxiang Liu, Daiqin Li, Xin Xu

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引用次数: 1

Abstract

ABSTRACT Accurate species delimitation is crucial for our understanding of evolution, biodiversity and conservation. However, morphology-based species delimitation alone appears to be prone to taxonomic errors and ineffective for taxa with high interspecific morphological homogeneity or intraspecific morphological variations, as is the case for mesothele and mygalomorph spiders. Combined molecular–morphology species delimitation has shown great potential to delimit species boundaries in such ancient lineages. In the present study, molecular and morphological evidence were integrated to delimit species of the primitively segmented spider genus Songthela Ono, 2000. The cytochrome c oxidase subunit I gene (COI) was sequenced for 192 novel specimens belonging to 12 putative morphospecies. The evolutionary relationships within Songthela and the 12-morphospecies hypothesis were tested in two steps – species discovery and species validation – using four single-locus species delimitation approaches. All species delimitation analyses supported the 12-species hypothesis. Phylogenetic analyses yielded three major clades in Songthela, which are consistent with morphology. Accordingly, we assigned 19 known and 11 new species (S. aokoulong, sp. nov., S. bispina, sp. nov., S. dapo, sp. nov., S. huayanxi, sp. nov., S. lianhe, sp. nov., S. lingshang, sp. nov., S. multidentata, sp. nov., S. tianmen, sp. nov., S. unispina, sp. nov., S. xiujian, sp. nov., S. zizhu, sp. nov.) of Songthela to three species-groups: the bispina-group, the multidentata-group and the unispina-group. Another new species, S. zimugang, sp. nov., is not included in any species groups, but forms a sister lineage to the bispina- and unispina-groups. These results elucidate a high species diversity of Songthela in a small area and demonstrate that integrating morphology with COI-based species delimitation is fast and cost-effective in delimiting species boundaries.

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综合分析表明，湖南原始分节蜘蛛属松蛛具有高度的物种多样性

摘要准确的物种划界对于我们理解进化、生物多样性和保护至关重要。然而，单独基于形态学的物种划界似乎容易出现分类学错误，对于具有高度种间形态同质性或种内形态变异的分类群来说是无效的，间皮蜘蛛和粘虫形态蜘蛛就是这样。分子-形态学相结合的物种划界显示出在这些古老谱系中划定物种边界的巨大潜力。在本研究中，分子和形态学证据被整合来界定原始节段蜘蛛属Songtela Ono的物种，2000年。对192个新标本的细胞色素c氧化酶亚基I基因（COI）进行了测序，这些标本属于12个假定的形态物种。Songtela内的进化关系和12种形态物种假说分两个步骤进行了测试——物种发现和物种验证——使用四种单位点物种划界方法。所有物种划界分析都支持12种假说。系统发育分析结果表明，松鸡属有三个主要的分支，它们与形态一致。因此，我们将松鸡属的19个已知物种和11个新种（澳口龙、双尖松鸡、大坡松鸡、花眼溪松鸡、连河松鸡、岭上松鸡、多齿松鸡、天门松鸡、unispina松鸡、秀尖松鸡和紫竹松鸡）分为三个类群：双尖松兔组，多齿组和单齿组。另一个新物种，S.zimugang，sp.nov.，不包括在任何物种群中，但形成了双锥虫和单锥虫群的姐妹谱系。这些结果阐明了Songtela在小面积内的高度物种多样性，并表明将形态学与基于COI的物种划界相结合在划定物种边界方面是快速且具有成本效益的。

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来源期刊

Invertebrate Systematics 生物-动物学

CiteScore

4.30

自引率

9.10%

发文量

审稿时长

>12 weeks

期刊介绍： Invertebrate Systematics (formerly known as Invertebrate Taxonomy) is an international journal publishing original and significant contributions on the systematics, phylogeny and biogeography of all invertebrate taxa. Articles in the journal provide comprehensive treatments of clearly defined taxonomic groups, often emphasising their biodiversity patterns and/or biological aspects. The journal also includes contributions on the systematics of selected species that are of particular conservation, economic, medical or veterinary importance. Invertebrate Systematics is a vital resource globally for scientists, students, conservation biologists, environmental consultants and government policy advisors who are interested in terrestrial, freshwater and marine systems. Invertebrate Systematics is published with the endorsement of the Commonwealth Scientific and Industrial Research Organisation (CSIRO) and the Australian Academy of Science.