Valorization of Waste Cooking Oil into Biodiesel via Bacillus stratosphericus Lipase Amine-Functionalized Mesoporous SBA-15 Nanobiocatalyst

IF 2.3 4区工程技术 Q3 ENGINEERING, CHEMICAL

International Journal of Chemical Engineering Pub Date : 2022-11-02 DOI:10.1155/2022/7899996

A. Ismail, Hamdy Kashtoh, M. A. Betiha, Salem A. Abu Amr, Kwan-Hyun Baek, N. El-Gendy

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引用次数: 1

Abstract

In this study, evaporation-induced self-assembly was applied to prepare amine-functionalized nano-silica (NH2-Pr-SBA-15). That was simply used to immobilize Bacillus stratosphericus PSP8 lipase (E–NH2–Pr-SBA-15), producing a nanobiocatalyst with good stability under vigorous shaking and a maximum lipase activity of 45 ± 2 U/mL. High-resolution X-ray diffractometer, Fourier transform infrared spectroscopy, N2 adsorption-desorption, field-emission scanning electron, and high-resolution transmission electron microscopic analyses proved the successful SBA-15 functionalization and enzyme immobilization. Response surface methodology based on a 1/2 fraction-three-levels face center composite design was applied to optimize the biodiesel transesterification process. This expressed efficient percentage conversion (97.85%) and biodiesel yield (97.01%) under relatively mild operating conditions: 3.12 : 1 methanol to oil ratio, 3.08 wt.% E–NH2–Pr-SBA-15 loading, 48.6°C, 3.19 h at a mixing rate of 495.53 rpm. E–NH2–Pr-SBA-15 proved to have a long lifetime, operational stability, and reusability.

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利用平流层芽孢杆菌脂肪酶胺功能化介孔SBA-15纳米生物催化剂催化废食用油制备生物柴油

本研究采用蒸发诱导自组装法制备胺功能化纳米二氧化硅(NH2-Pr-SBA-15)。将其简单地固定化平流层芽孢杆菌PSP8脂肪酶(E-NH2-Pr-SBA-15)，得到了在剧烈摇动下稳定性良好的纳米生物催化剂，最大脂肪酶活性为45±2 U/mL。高分辨率x射线衍射仪、傅里叶变换红外光谱、N2吸附-解吸、场发射扫描电子和高分辨率透射电镜分析证实了SBA-15的成功功能化和酶固定化。采用基于1/2分数-三级面中心复合设计的响应面法对生物柴油的酯交换工艺进行了优化。在相对温和的操作条件下，转化率(97.85%)和生物柴油收率(97.01%)为:3.12:1的甲醇与油比，3.08 wt.%的E-NH2-Pr-SBA-15负载，48.6°C, 3.19 h，混合速率为495.53 rpm。事实证明，E-NH2-Pr-SBA-15具有较长的使用寿命、运行稳定性和可重用性。

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来源期刊

International Journal of Chemical Engineering Chemical Engineering-General Chemical Engineering

CiteScore

4.00

自引率

3.70%

发文量

审稿时长

14 weeks

期刊介绍： International Journal of Chemical Engineering publishes papers on technologies for the production, processing, transportation, and use of chemicals on a large scale. Studies typically relate to processes within chemical and energy industries, especially for production of food, pharmaceuticals, fuels, and chemical feedstocks. Topics of investigation cover plant design and operation, process design and analysis, control and reaction engineering, as well as hazard mitigation and safety measures. As well as original research, International Journal of Chemical Engineering also publishes focused review articles that examine the state of the art, identify emerging trends, and suggest future directions for developing fields.