Spray-induced gene silencing: an innovative strategy for plant trait improvement and disease control

IF 1.5 4区农林科学 Q2 Agricultural and Biological Sciences

Crop Breeding and Applied Biotechnology Pub Date : 2021-07-30 DOI:10.1590/1984-70332021v21sa24

R. Vetukuri, M. Dubey, P. Kalyandurg, A. Carlsson, S. Whisson, R. Ortiz

引用次数: 11

Abstract

Abstract Modern plant breeding is still a time-consuming and costly process, even with the most advanced technologies such as gene editing. Hence, there is an urgent need to develop alternative means for plant trait manipulation and plant protection. RNA interference (RNAi) is a conserved cellular mechanism mediated by naturally occurring double-stranded RNA (dsRNA) and small RNAs (sRNAs) that can target mRNAs for destruction or transcript reduction. Here, we review the potential of technology based on RNAi, called spray-induced gene silencing (SIGS), as an alternative or adjunct to breeding for manipulation of endogenous gene expression in plants or pathogen control. SIGS based on exogenous application of RNA molecules in plants may be especially useful in reducing pest or pathogen impacts, thereby ameliorating biotic stresses and increasing the agronomic performance of crops.

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喷雾诱导基因沉默：植物性状改良和病害控制的创新策略

现代植物育种仍然是一个耗时和昂贵的过程，即使有最先进的技术，如基因编辑。因此，迫切需要开发植物性状操纵和植物保护的替代手段。RNA干扰(RNAi)是由天然存在的双链RNA (dsRNA)和小RNA (sRNAs)介导的一种保守的细胞机制，可以靶向mrna进行破坏或转录物减少。在这里，我们回顾了基于RNAi技术的潜力，称为喷雾诱导基因沉默(SIGS)，作为一种替代或辅助育种，用于操纵植物内源基因表达或病原体控制。基于外源RNA分子在植物中的应用的SIGS可能在减少害虫或病原体的影响，从而改善生物胁迫和提高作物的农艺性能方面特别有用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Crop Breeding and Applied Biotechnology 农林科学-农艺学

CiteScore

2.40

自引率

13.30%

发文量

审稿时长

6-12 weeks

期刊介绍： The CBAB – CROP BREEDING AND APPLIED BIOTECHNOLOGY (ISSN 1984-7033) – is the official quarterly journal of the Brazilian Society of Plant Breeding, abbreviated CROP BREED APPL BIOTECHNOL. It publishes original scientific articles, which contribute to the scientific and technological development of plant breeding and agriculture. Articles should be to do with basic and applied research on improvement of perennial and annual plants, within the fields of genetics, conservation of germplasm, biotechnology, genomics, cytogenetics, experimental statistics, seeds, food quality, biotic and abiotic stress, and correlated areas. The article must be unpublished. Simultaneous submitting to another periodical is ruled out. Authors are held solely responsible for the opinions and ideas expressed, which do not necessarily reflect the view of the Editorial board. However, the Editorial board reserves the right to suggest or ask for any modifications required. The journal adopts the Ithenticate software for identification of plagiarism. Complete or partial reproduction of articles is permitted, provided the source is cited. All content of the journal, except where identified, is licensed under a Creative Commons attribution-type BY. All articles are published free of charge. This is an open access journal.