Neuroprotective Activity of Blepharis maderaspatensis Against Colchicine Induced Alzheimer’s Disease Rat Model

Abstract

Traditionally, plants have been used for various diseases and neuronal disorders. The present study was designed to evaluate the neuroprotective effect of Blepharis maderaspatensis against a colchicine-induced Alzheimer’s disease rat model. Background and Objective: Traditionally, plants were used for various diseases and neuronal disorders. The present study was designed to evaluate the neuroprotective effect of Blepharis maderaspatensis against colchicine induced Alzheimer’s disease rat model. Neuroprotective activity was examined on colchicine-induced Alzheimer’s disease rats. Test group animals were administrated with ethanol extract of Blepharis maderaspatensis at doses of 200, 400, and 600mg/kg for 28 days. The behavioral parameters were assessed using the Morris water maze and a Digital actophotometer at days 0, 7, 14, and 28 on intracerebroventricular administration of colchicine for assessing the cognitive and gross behavioral activities in rats. Biochemical parameters like lipid peroxidation, glutathione, superoxide dismutase, catalase, total thiols, and acetylcholinesterase were assessed and histopathology of brain tissue was examined on the final day of the experiment. Ethanol extract of Blepharis maderaspatensis showed a dose-dependent neuroprotective activity with a significant decrease in time latency of the Morris water maze (p<0.001) and an increase in the locomotor activity (p<0.001) in the test group as compared to the control group. Biochemical parameters showed a significant decrease in lipid peroxidation (p<0.001) and acetylcholinesterase level (p<0.001) and an increase in superoxide dismutase (p<0.001), catalase (p<0.001), glutathione (p<0.001), and total thiols (p<0.001) in the test group as compared to the control group. In conclusion, the present study showed that the administration of ethanol extract of Blepharis maderaspatensis exerted neuroprotective activity against colchicine-induced cognitive impairment and associated oxidative stress. Ethanol extract of Blepharis maderaspatensis showed a dose dependent neuroprotective activity with significant decrease in time latency of Morris water maze (p<0.001) and increase in the locomotor activity (p<0.001) in test group as compared to control group. Biochemical parameters showed significant decreases in lipid peroxidation (p<0.001), acetylcholinesterase level (p<0.001) and increased in superoxide dismutase (p<0.001) catalase (p<0.001), glutathione (p<0.001) total thiols (p<0.001) in the test group as compared control group. In conclusion, the present study showed that administration of ethanol extract of Blepharis maderaspatensis possesses neuroprotective activity against colchicine induced cognitive impairment and associated oxidative stress.