MOLECULAR SCREENING OF BASAL STEM ROT RESISTANCE GENES IN OIL PALM

IF 1.3 4区农林科学 Q2 Agricultural and Biological Sciences

Journal of Oil Palm Research Pub Date : 2022-12-23 DOI:10.21894/jopr.2022.0078

Mohd Amin AB-HALIM

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Abstract

Basal stem rot (BSR) is one of the main oil palm diseases that have led to tremendous losses in oil yields for almost a century. Having markers, especially those that are linked to resistance (R) genes could potentially alleviate this problem by providing the tools to select palms that are tolerant or resistant to the disease. This study aimed to develop oil palm genomic markers that can distinguish oil palm plants with different levels of resistance to BSR. We identified 144 homologous R genes in the oil palm genome based on the conserved domain structure of known R proteins. Six simple sequence repeat markers were identified and used to genotype 40 palms with different levels of resistance to BSR. The observed and effective number of alleles ranged from 2 to 7 and 1.57 to 4.30, respectively. The observed heterozygosity ranged from 0.13 to 0.67, with a mean of 0.46 while the expected heterozygosity ranged from 0.40 to 0.78, with a mean of 0.51. Analysis of genetic distances from the set of markers was able to differentiate susceptible and tolerant palm samples. These results may help in the early selection of durable BSR disease resistant oil palm cultivars.

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油棕基茎腐病抗性基因的分子筛选

近一个世纪以来，基底茎腐病是造成油棕产量巨大损失的主要油棕病害之一。有了标记，特别是那些与抗性(R)基因相关的标记，可以通过提供工具来选择对该疾病具有耐受性或抗性的棕榈树，从而潜在地缓解这一问题。本研究旨在开发油棕基因组标记，以区分不同抗BSR水平的油棕植株。基于已知R蛋白的保守结构域结构，我们在油棕基因组中鉴定出144个同源R基因。鉴定了6个简单序列重复标记，并对40株不同程度BSR抗性的棕榈进行了基因分型。观察等位基因数为2 ~ 7个，有效等位基因数为1.57 ~ 4.30个。观察到的杂合度范围为0.13 ~ 0.67，平均为0.46;期望杂合度范围为0.40 ~ 0.78，平均为0.51。从一组标记的遗传距离分析能够区分易感和耐受性棕榈样品。这些结果可能有助于早期选择耐BSR病的油棕品种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Oil Palm Research 农林科学-食品科技

CiteScore

2.60

自引率

30.80%

发文量

审稿时长

>12 weeks

期刊介绍： JOURNAL OF OIL PALM RESEARCH, an international refereed journal, carries full-length original research papers and scientific review papers on various aspects of oil palm and palm oil and other palms. It also publishes short communications, letters to editor and reviews of relevant books. JOURNAL OF OIL PALM RESEARCH is published four times per year, i.e. March, June, September and December.