Metastatic calcification and granulomatous grastroenteritis associated to Pythium insidiosum in a dog

Q4 Veterinary
Isabela F. S. Perossi, M. Manco, S. Ferreira, M. Klein, Marina Emanoella Seruti Pelógia, G. Carra, C. Souza, M. T. Costa, S. Bosco, P. Moraes, R. Vasconcelos
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引用次数: 2

Abstract

Pythiosis is a granulomatous process of which the oomycete Pythium insidiosum is its etiological agent. It can affect animals and humans alike and its infection occurs when free zoospores in the water get in contact with the target tissues and encyst. The disease often occurs in tropical places with abundance of water and aquatic plants that host the fungus. Dogs infection is predominantly gastric with granuloma formations in the stomach and intestine with progressive signs of vomiting, weight loss and diarrhea. In this case report, we described clinical, surgical, necroscopic and histopathological findings of a one year and two months old, male boxer that presented clinical signs of anorexia and persistent vomiting. It was noticed on ultrasound examination an increase in stomach and intestine thickness. Laparotomy confirmed a mass affecting the gastric wall which, an incision biopsy, showed an abundant fibrous tissue associated with granulomatous reaction that was surrounded by tubuliform structures. Due to clinical complications, euthanasia was performed and in necroscopic examination a markedly increased stomach and duodenum was observed. An; histological examination of this areas it was observed that they contained granulation tissue with giant cells and epithelioids macrophages around necrosed areas associated with lymphocytes infiltrate. Also, it was possible to observe tubuliform structures by the Grocott-Gomori’s Methenamine Silver (GMS) stain, this finding is compatible with the agent Pythium insidiosum. Therefore, this presumptive identification was confirmed by PCR analysis which amplicon had 97.83% similarity with current available genomic sequence of P. insidiosum.
转移性钙化和肉芽肿性肠胃炎与狗的皮癣有关
Pythiosis是一种肉芽肿性过程,其病原体是卵霉菌Pythium insidiosum。它可以影响动物和人类,当水中的游离游动孢子接触到目标组织和囊时,就会感染。这种疾病经常发生在热带地区,那里有丰富的水和水生植物,它们是真菌的宿主。狗的感染主要是胃,胃和肠中形成肉芽肿,伴有呕吐、体重减轻和腹泻的渐进症状。在本病例报告中,我们描述了一名一岁零两个月大的男性拳击手的临床、手术、尸检和组织病理学结果,该拳击手表现出厌食症和持续呕吐的临床症状。超声检查发现胃和肠的厚度增加。剖腹产证实有一个影响胃壁的肿块,切开活检显示有大量与肉芽肿反应相关的纤维组织,周围有管状结构。由于临床并发症,实施了安乐死,在尸检中观察到胃和十二指肠明显增加。一对该区域的组织学检查观察到,在与淋巴细胞浸润相关的坏死区域周围,它们包含具有巨细胞和上皮样巨噬细胞的肉芽组织。此外,通过Grocott-Gomori的Methenamine Silver(GMS)染色可以观察到管状结构,这一发现与Pythium insidiosum试剂相容。因此,通过PCR分析证实了这一推定鉴定,该扩增子与隐孢子虫目前可用的基因组序列具有97.83%的相似性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Brazilian Journal of Veterinary Pathology
Brazilian Journal of Veterinary Pathology Veterinary-Veterinary (miscellaneous)
CiteScore
0.60
自引率
0.00%
发文量
24
期刊介绍: The Brazilian Journal of Veterinary Pathology is the official electronic periodical of the Brazilian Association of Veterinary Pathology. The purpose of the BJVP is to publish original full papers, short communications, case reports, letters, reviews (by invited experts) and abstracts of scientific meetings. The preferable subjects is natural and experimental pathology. All the articles are submitted to scientific reviewers.
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