Leishmanicidal Effects of Allium giganteum Saponin-Rich Fraction on Leishmania major

IF 1.1 Q4 PHARMACOLOGY & PHARMACY
Omid Changiz, Abbasali Eskandarain, Masoud Sadeghi-Dinani, S. Soleimanifard
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引用次数: 1

Abstract

Background and objectives: Leishmaniasis is caused by the genus of Leishmania and is one of the important health problems worldwide. Serious side effects, the lack of effective vaccines and the emergence of drug resistance are the major weak points of leishmaniasis treatment. The purpose of this study was to evaluate leishmanicidal effects of Allium giganteum saponin rich fraction, natural compounds with history of antimicrobial properties, on promastigotes and axenic amastigotes of L. major and macrophages cell line J774. Methods: The chloroform-methanol (9:1) extract of the flowers was fractionated by MPLC using an RP-18 column. The saponin-rich fraction was detected by TLC and H-NMR analyses and evaluated for leishmanicidal activity on L. major and macrophages cell line J774 using MTT assay at 24, 48 and 72 h of incubation. Results: At concentrations of 75, 100 and 150 μg/mL, over the time of 24 to 72 h, a significant decrease in the live parasite's rate was observed (p <0.05). At 200 μg/mL concentration, all parasites were killed and maximum leishmanicidal effect was observed. The IC50s for promastigotes and axenic amastigotes were 90.01 ± 13.42 μg/mL and 29.76±17.91 μg/mL, respectively; the value for the J774 macrophage cell line was 33.17±4 μg/mL. Conclusion: The results of this study showed the significant leishmanicidal effect of saponin rich fraction from Allium giganteum on promastigote and axenic amastigote of L. major and macrophage cell line in vitro. Complementary in vivo studies for evaluating the effects of the fraction on leishmaniasis in BALB/c mice is recommended.
葱总皂苷提取物对大利什曼原虫的杀虫作用
背景与目的:利什曼病是由利什曼原虫属引起的,是世界范围内重要的健康问题之一。严重的副作用、缺乏有效的疫苗和出现耐药性是利什曼病治疗的主要弱点。本研究的目的是评估富含大葱皂苷的部分(具有抗菌历史的天然化合物)对L.major和巨噬细胞系J774的前鞭毛虫和无菌无鞭毛虫的杀利什曼原虫作用。方法:采用RP-18色谱柱,用MPLC对花的氯仿-甲醇(9:1)提取物进行分级。通过TLC和H-NMR分析检测富含皂苷的组分,并在培养24、48和72小时时使用MTT分析评估对L.major和巨噬细胞细胞系J774的利什曼原虫活性。结果:在75、100和150μg/mL浓度下,在24至72小时内,观察到活寄生虫率显著下降(p<0.05)。在200μg/mL的浓度下,所有寄生虫都被杀死,并观察到最大的利什曼原虫杀灭效果。前鞭毛体和无菌无鞭毛体的IC50分别为90.01±13.42μg/mL和29.76±17.91μg/mL;μg/mL。结论:大葱富含皂苷组分对体外培养的L.major和巨噬细胞系的前鞭毛体和无菌无鞭毛体具有显著的利什曼原虫杀灭作用。建议进行补充体内研究,以评估该部分对BALB/c小鼠利什曼病的影响。
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来源期刊
Research Journal of Pharmacognosy
Research Journal of Pharmacognosy PHARMACOLOGY & PHARMACY-
CiteScore
1.10
自引率
20.00%
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0
审稿时长
8 weeks
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