The Effect of the Extracts of Endophytic Fungi on Pancreatic α-Amylase Activity

食品科学与工程:英文版(美国) Pub Date : 2017-10-28 DOI:10.17265/2159-5828/2017.10.006

D. Ruzieva, H. Hasanov, L. Abdulmyanova, G. Rasulova, R. Sattarova, T. Gulyamova

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引用次数: 3

Abstract

Inhibitors of pancreatic α-amylase offer an effective strategy to lower the levels of postprandial hyperglycemia by control of starch breakdown. Among 86 fungal endophytes isolated from 15 medicinal plants Aspergillus terreus-AF104S, Aspergillus egypticus-HT166S and Penicillium sp.-CC200 exhibited strong pancreatic amylase inhibitory potential were selected. Endophytes were subjected to ethyl acetate extraction and tested for α-amylase inhibition, in order to assess and evaluate their inhibitory potential on pancreatic α-amylase. Analysis showed concentration dependent enzyme inhibition up to 83% with half inhibition (IC50) values for less 25 mg⋅mL, which is lower than acarbose as control. It was observed 3-fold increasing of Vmax and maintenance Km at control level in the presence of extracts A. terreus-AF104S and Penicillium sp.-CC200, while in presence of extract A. egypticus-HT166S Km was doubled, and Vmax was maintained at the control level. Kinetic studies allow proposing the competitive mode of α-amylase inhibition by extracts A. egypticus-HT166S and uncompetitive inhibition by extracts A. terreus-AF104S and Penicillium sp.-CC200.

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内生真菌提取物对胰腺α-淀粉酶活性的影响

胰腺α-淀粉酶抑制剂提供了一种通过控制淀粉分解来降低餐后高血糖水平的有效策略。从15种药用植物中分离到86株真菌内生菌，其中土曲霉-AF104S、埃及曲霉-HT166S和青霉属。对内生植物进行乙酸乙酯提取，并测试其对α-淀粉酶的抑制作用，以评估和评估其对胰腺α-淀粉酶抑制的潜力。分析显示，浓度依赖性酶抑制率高达83%，25 mg·mL以下的半数抑制（IC50）值低于阿卡波糖作为对照。在A.terreus-AF104S和Penicillium sp.-CC200提取物存在下，观察到Vmax和维持Km在对照水平上增加了3倍，而在A.egypticus-HT166S提取物存在下Km增加了一倍，并且Vmax维持在对照水平。动力学研究允许提出埃及A.egypticus-HT166S提取物抑制α-淀粉酶的竞争模式，以及A.terreus-AF104S和Penicillium sp.-CC200提取物的无竞争抑制模式。

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来源期刊

食品科学与工程:英文版(美国)

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