Evaluation of the Protective Effect of The Cup Therapy on the Epileptic Seizure in Rats

Abstract

Objective: Cup therapy has an important place in traditional and complementary medicine applications. The purpose of our study, this is the first time to investigate the protective effect of cup therapy in rats on experimentally generated epileptic seizures in new rat modeling created by different anatomic regions. Methods: A total of 42 Wistar albino rats were randomly divided into 6 groups (n:7). The "new dry cup" was applied to the G1 group, and the "new wet cup" model was applied to the G2. In the G3 group, an "epilepsy model" (PTZ, 35 mg / kg) was created and ''diazepam'' (2.5 mg / kg) was given to G4. "Dry cup" and "wet cup" models were applied to the G5 and G6 groups, respectively. 24 hours after the cupping therapies, the rats were injected with PTZ and the epilepsy behavior scores of the rats in all groups were recorded for 20-30 minutes. Results: In the 'Open Area' and 'Elevated Plus Maze' tests, there was no behavioral difference between the cup therapy group and the control group (p > 0.05). Given all the parameters, the G4 group significantly reduces the seizure compared to other groups (p < 0.05). There is a significant difference in G2, G5 and G6 groups compared to G3 in the phases parameter (p < 0.05). Conclusions: In this study, the new wet cup therapy (G2), which was applied for the first time, had a protective effect on seizures. G2, G5 and G6 groups are observed to suppress seizures compared to G3. Our findings are expected to contribute greatly to animal model analysis in the future.