Epithelial Cells Transfer: An Experimental Study

A. Piters, A. Esponda, G. Monique, A. Doutremepuich, S. Fromentin, C. Doutremépuich
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Abstract

Objective: The objective of the present study was to analyze the conditions of epithelial cells transfer after an initial deposition on a first object to a second object (secondary transfer). This study should provide an experimental model which should contribute to better understand and analyze all the transfer mechanisms. Methods: Using glass slides and cotton gauzes as supports for the transfer of epithelial cells from an object to another one, we analyzed the occurrence and efficiency of cells transfer in different conditions of hygrometry and contact. Results: Our investigation reveals that after one hour deposition on a glass slide, the epithelial cells are no longer able to transfer to a second support. Moreover, our results also demonstrate that in opposition with the environmental factors such as hygrometry which are determinant for the transfer mechanisms, the strength of the contact between the glass slide and cotton gauze does not influence the cells transfer efficiency. Conclusion: In conclusion, the secondary transfer of epithelial cells is only possible if the cells are not strongly attached to their support.
上皮细胞转移的实验研究
目的:本研究的目的是分析上皮细胞在第一个物体上初次沉积到第二个物体(二次转移)后的转移条件。这项研究应该提供一个实验模型,有助于更好地理解和分析所有的转移机制。方法:用载玻片和棉网作为载体,将上皮细胞从一个物体转移到另一个物体,我们分析了不同湿度和接触条件下细胞转移的发生率和效率。结果:我们的研究表明,在载玻片上沉积一小时后,上皮细胞无法再转移到第二个支架上。此外,我们的结果还表明,与决定转移机制的环境因素(如湿度计)相反,载玻片和棉布之间的接触强度不会影响细胞转移效率。结论:总之,只有当上皮细胞没有牢固地附着在其支架上时,上皮细胞的二次转移才有可能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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