Simultaneous detection of purine metabolites by membrane modified electrochemical sensors

IF 0.9 Q4 CHEMISTRY, MULTIDISCIPLINARY
J. Blaskovicová, Andrea Purdesová
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引用次数: 1

Abstract

Abstract Purine metabolites are important for metabolic and cellular processes. Deregulation of purinergic signaling leads to pathological accumulation of purine degradation products in extracellular fluids and indicates various diseases. In clinical diagnosis at early stages of related diseases, accurate detection of Uric acid and Xanthine is of high importance. Electrochemical methods are fast, simple, sensitive, more convenient, and cost-effective compared to other analytical methods used in purine metabolites signaling. Electrochemical sensors are able to detect more compounds simultaneously. Modification of a glassy carbon electrode sensor with external protective membranes was used in this study to avoid unwanted signal interferences from analyte matrices. Polyvinyl alcohol, Chitosan, and Nafion membranes were selected for sensor modification to compare the electro-neutral, positive and negative charged setting of the Xanthine and Uric acid detection. All three membrane modified sensors showed adequate stability in the phosphate buffer solution after 5 min of incubation and are thus suitable for simultaneous detection of purine metabolites. The best results in anodic peak current response values were observed using the Nafion membrane modified glassy carbon electrode sensor. The approach reported here can be useful for the detection of purine metabolites from various matrices at early stages of clinical diagnosis.
膜修饰电化学传感器同时检测嘌呤代谢产物
摘要嘌呤代谢产物对代谢和细胞过程很重要。嘌呤能信号的失调导致嘌呤降解产物在细胞外液中的病理性积累,并指示各种疾病。在相关疾病的早期临床诊断中,准确检测尿酸和黄原酸具有重要意义。与嘌呤代谢产物信号传导中使用的其他分析方法相比,电化学方法快速、简单、灵敏、更方便、成本效益高。电化学传感器能够同时检测更多的化合物。本研究中使用了带有外部保护膜的玻璃碳电极传感器的改进,以避免分析物基质的不必要信号干扰。选择聚乙烯醇、壳聚糖和Nafion膜进行传感器修饰,以比较黄原酸和尿酸检测的电中性、正电荷和负电荷设置。所有三种膜修饰的传感器在培养5分钟后在磷酸盐缓冲溶液中显示出足够的稳定性,因此适合于同时检测嘌呤代谢产物。使用Nafion膜修饰的玻碳电极传感器观察到阳极峰值电流响应值的最佳结果。本文报道的方法可用于在临床诊断的早期阶段检测各种基质中的嘌呤代谢产物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta Chimica Slovaca
Acta Chimica Slovaca CHEMISTRY, MULTIDISCIPLINARY-
自引率
12.50%
发文量
11
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