Assessment of DNA damage in lymphocytes of Mamuju (a high background radiation area) inhabitants using alkaline single cell gel electrophoresis

Q4 Medicine
Tur Rahardjo, W. Mailana, T. Kisnanto, Darlina, S. Nurhayati, D. Tetriana, D. Ramadhani, M. Syaifudin
{"title":"Assessment of DNA damage in lymphocytes of Mamuju (a high background radiation area) inhabitants using alkaline single cell gel electrophoresis","authors":"Tur Rahardjo, W. Mailana, T. Kisnanto, Darlina, S. Nurhayati, D. Tetriana, D. Ramadhani, M. Syaifudin","doi":"10.1504/IJLR.2017.10008590","DOIUrl":null,"url":null,"abstract":"Mamuju in Indonesia has a highest average dose rate and considered as a high background radiation area (HBRA) in Indonesia. Here in this research a preliminary study to evaluate DNA damage using the alkaline comet assay in inhabitants of Takandeang village, Mamuju, was conducted. Blood samples from 55 healthy donors living in Takandeang village and 22 control samples were obtained and processed into the assay. Results showed that there were insignificant differences of comet tail length (TL) and tail moment (TM) between Takandeang village inhabitants and control samples (p = 0.578, 0.174). Regression analysis revealed that the DNA damage was increased with age in control samples, even though the correlation was not significant (p > 0.05). In contrast a significant negative correlation (p = 0.02) was observed in Takandeang inhabitants. Results found in this research should be validating in a larger study using more samples from Mamuju.","PeriodicalId":14141,"journal":{"name":"International Journal of Low Radiation","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2017-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"International Journal of Low Radiation","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1504/IJLR.2017.10008590","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 3

Abstract

Mamuju in Indonesia has a highest average dose rate and considered as a high background radiation area (HBRA) in Indonesia. Here in this research a preliminary study to evaluate DNA damage using the alkaline comet assay in inhabitants of Takandeang village, Mamuju, was conducted. Blood samples from 55 healthy donors living in Takandeang village and 22 control samples were obtained and processed into the assay. Results showed that there were insignificant differences of comet tail length (TL) and tail moment (TM) between Takandeang village inhabitants and control samples (p = 0.578, 0.174). Regression analysis revealed that the DNA damage was increased with age in control samples, even though the correlation was not significant (p > 0.05). In contrast a significant negative correlation (p = 0.02) was observed in Takandeang inhabitants. Results found in this research should be validating in a larger study using more samples from Mamuju.
碱性单细胞凝胶电泳评价马木居(高本底辐射区)居民淋巴细胞DNA损伤
印度尼西亚马穆朱的平均剂量率最高,被认为是印度尼西亚的高本底辐射区。在本研究中,对Mamuju Takandeang村居民进行了一项使用碱性彗星测定法评估DNA损伤的初步研究。从居住在Takandeang村的55名健康献血者和22个对照样本中获得血液样本,并将其加工成测定方法。结果表明,Takandeang村居民的彗尾长度(TL)和彗尾力矩(TM)与对照组差异不显著(p = 0.578, 0.174)。回归分析显示,在对照样本中,DNA损伤随年龄的增长而增加,但相关性不显著(p < 0.05)。相反,在Takandeang居民中观察到显著的负相关(p = 0.02)。这项研究的结果应该在使用更多来自Mamuju的样本的更大规模的研究中得到验证。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
International Journal of Low Radiation
International Journal of Low Radiation Environmental Science-Health, Toxicology and Mutagenesis
自引率
0.00%
发文量
0
期刊介绍: The IJLR is a peer-reviewed journal dedicated to the publication of research articles, review papers and technical notes in all domains related to low-dose radiation, among which are the biological and health effects in humans and the biota, in vitro and in vivo research on low radiation effects, regulatory and policy aspects, risk estimation and public perception.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信