Study on microflora structure of Macrobrachium rosenbergii larvae and environmental water at different developmental stages

Abstract

Abstract 16S rRNA gene sequencing was used to analyze the changes in the microbial community structure of Macrobrachium rosenbergii (M. rosenbergii) larvae and environmental water at different stages (0, 7, 14 and 21 days). The PICRUSt was used to precalculated the predicted genes and their functions. The Alpha and Beta diversity analysis showed that the abundance and uniformity of the flora of larvae and water samples were significantly different at four stages. At the phylum level, Proteobacteria, Planctomycetes, Firmicutes and Bacteroidetes were the dominant flora of larvae, while Proteobacteria, Bacteroidetes and Actinobacteria were the dominant flora of environmental water. But at the genus level, Raoultella and Blastopirellula were the main changes in larvae, and Dyadobacter, Flavobacterium and Pseudomonas were the main changes in environmental water. The gene function prediction analysis indicated that the amino acid metabolism, immune system, immune system disease, nervous system, sensory system and other pathways enriched in the different stages of larvae and environmental water were closely related to the breeding of M. rosenbergii. This research might provide theoretical basis and guidance for the high-quality breeding of M. rosenbergii. HIGHLIGHTS The abundance and uniformity of the flora of larvae and environmental water were significantly different at different stages. Several flora were found that related to the growth and development of larval. KEGG pathways enriched at different stages of larvae and environmental water were closely related to the breeding of larvae.