In vitro Antagonistic Activity of Diverse Bacillus Species Against Cochliobolus sativus (Common Root Rot) of Barley

Acta Phytopathologica Et Entomologica Hungarica Pub Date : 2020-08-31 DOI:10.1556/038.55.2020.012

M. Harba, M. Jawhar, M. Arabi

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引用次数: 3

Abstract

Antagonistic microorganisms have been used as biological control agents to reduce the use of chemical fungicides in the control of crop diseases. The present work was conducted to determine the antagonistic potential of Bacillus sp. isolates against the soil-borne fungus Cochliobolus sativus, the causal agent of barley common root (CRR) disease. Out of 525 isolates, 40 were showed in vitro antagonistic activity against the virulent C. sativus isolate CRR16. On the basis of 16S rRNA gene sequencing Bacillus sp. isolates are identified as B. atrophaeus, B. subtilis, Paenibacillus polymyxa, B. amyloliquefaciens, B. simplex and B. tequilensis. Results showed that Bacillus sp. had significant (P<0.05) antagonistic activities against the C. sativus where the percentage of radial growth inhibition of the fungi colonies ranged from 59 to 92%, compared to the untreated control. The B. subtilis isolate SY41B had the highest inhibition effect on the vegetative growth zones as compared with the other species isolates. In view of these, our results indicate that the antagonistic effect of the Bacillus sp. isolates may be important contributors as a biocontrol approach that could be employed as a part of integrated CRR management system.

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不同芽孢杆菌对大麦根腐病的体外拮抗作用

拮抗微生物已被用作生物防治剂，以减少化学杀菌剂在控制作物疾病中的使用。本工作旨在确定芽孢杆菌分离株对大麦根腐病病原土传真菌Cochliobolus sativus的拮抗潜力。在525个分离株中，有40个在体外表现出对强毒的C.sativus分离株CRR16的拮抗活性。根据16S rRNA基因测序，芽孢杆菌分离株被鉴定为萎缩芽孢杆菌、枯草芽孢杆菌、多粘Paenibacillus polymyxa、解淀粉芽孢杆菌、单纯芽孢杆菌和龙舌兰芽孢杆菌。结果表明，与未处理的对照相比，芽孢杆菌对C.sativus具有显著的拮抗活性（P<0.05），其中真菌菌落的径向生长抑制率在59%至92%之间。枯草芽孢杆菌菌株SY41B对营养生长区的抑制作用最高。鉴于此，我们的研究结果表明，芽孢杆菌的拮抗作用可能是一种重要的生物控制方法，可作为综合CRR管理系统的一部分。

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来源期刊

Acta Phytopathologica Et Entomologica Hungarica Agricultural and Biological Sciences-Plant Science

CiteScore

1.10

自引率

0.00%

发文量

期刊介绍： The journal publishes papers on the infectious diseases of plants, damages caused by insects and deals with the basic aspects of chemical and biological protection. Within its field of interest additional topics such as resistance against plant diseases, and physiological, biochemical and molecular questions of plant resistance and susceptibility are also discussed. Publishes book reviews and advertisements.