Melanocortin 1 receptor (MC1R) gene polymorphism and its association with coat colour patterns in Nagpuri and Purnathadi buffaloes

IF 0.2 4区农林科学 Q4 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Buffalo Bulletin Pub Date : 2022-09-29 DOI:10.56825/bufbu.2022.4133555

Madhuri Kamble, S. Kuralkar, Syed Sajid Ali, Pravin Suryakant Bankar, Prajakta Kuralkar, S. Deshmukh

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Abstract

Melanin is the pigment of skin (coat) and hairs in all mammals. The 954 bp long MC1R gene codes for the production of MC1R or the α-MSH receptors which are present on the melanosomes. These receptors regulate the synthesis of melanin pigments viz., eumelanin or phaeomelanin leading to black and reddish to yellowish pigmentations respectively. PCR-RFLP analysis of 138 bp region of MC1R gene was conducted in two buffalo populations having distinct coat colours; Nagpuri (black) and Purnathadi (whitish). Three genotypes, EDED, EDE* and E*E* were observed at frequencies of 0.86, 0.03 and 0.11 for Nagpuri and 0.74, 0.08 and 0.18 in Purnathadi buffaloes; similarly the observed gene frequencies for ED and E* alleles were 0.87, 0.13 and 0.78, 0.22 in Nagpuri and Purnathadi buffaloes, respectively. DNA-sequencing revealed a transition mutation (A→G) at 118 bp locus confirming polymorphism, however presence in both the population failed to point towards any specific role in body coat colour of buffaloes.

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Nagpuri和Purnathadi水牛MC1R基因多态性及其与毛色模式的关系

黑色素是所有哺乳动物皮肤（皮毛）和毛发的色素。954bp长的MC1R基因编码MC1R或存在于黑素体上的α-MSH受体的产生。这些受体调节黑色素的合成，即真黑色素或淡黑色素，分别导致黑色和红色至黄色。对具有不同毛色的两个水牛群体的MC1R基因138bp区域进行了PCR-RFLP分析；Nagpuri（黑色）和Purnathadi（白色）。Nagpuri水牛的EDED、EDE*和E*E*三种基因型频率分别为0.86、0.03和0.11，Purnathadi水牛的频率分别为0.74、0.08和0.18；同样，在Nagpuri和Purnathadi水牛中观察到的ED和E*等位基因频率分别为0.87,0.13和0.78,0.22。DNA测序显示一个过渡突变（a→G）在118bp的基因座上证实了多态性，但在这两个群体中的存在并没有表明水牛体毛颜色的任何特定作用。

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来源期刊

Buffalo Bulletin AGRICULTURE, DAIRY & ANIMAL SCIENCE-

CiteScore

0.50

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Buffalo Bulletin is published quarterly in January-March, April-June, July-September and October-December. Contributions on any aspect of research or development, progress reports of projects and news on buffalo will be considered for publication in the bulletin.