Hydrocarbon Utilizing and Metal Tolerant Bacteria Simultaneously Degrade Hydrocarbons and Detoxify Metals in Petroleum Contaminated Soil

IF 2.2 4区环境科学与生态学 Q3 ENVIRONMENTAL SCIENCES

Geomicrobiology Journal Pub Date : 2023-02-15 DOI:10.1080/01490451.2023.2176573

P. A. Wani, Adebimpe Nusirat Ebudola, Y. K. Olusebi, N. Rafi, Sanusi Jadesola Fawzhia O, Oyelami Isaac Oluwaseun

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引用次数: 2

Abstract

Abstract Petroleum contamination to our biosphere particularly soil and water is due to release of petroleum products from petrochemical industries and is now a day’s biggest problem to the mankind. Present work was conducted to assess capability of hydrocarbon-utilizing and metal tolerant bacteria to degrade hydrocarbons and remediate metals in the soil contaminated with petroleum with the aim to form super bio-inoculants to restore soil to its original state. In order to achieve above goal, petroleum contaminated soil was observed for hydrocarbons using GC-MS, a total of 25 hydrocarbons were found in petroleum polluted soil with hydrocarbon concentration of 3738 mg/kg. Petroleum polluted soil was also observed for total bacterial population which was observed as 2.10 × 106 CFU/g of soil. Strains YSA-7 and YSA-8 showed highest potential for hydrocarbon-utilization with an absorbance of 0.681 and 0.614 respectively. Strains were also highly tolerant to Cu and Zn with tolerance of 600 µg/ml (Cu) and 1500 µg/ml (Zn) by strain YSA-7 and 700 µg/ml (Cu) and 1800 (Zn) µg/ml by strain YSA-8. Strains YSA-7 and YSA-8 were identified by 16 SrRNA as Lysinibacillus fusiformis and Stenotrophomonas maltophilia, respectively. After 20 days of incubation, Lysinibacillus fusiformis strain YSA-7 and Stenotrophomonas maltophilia YSA8 degraded all twenty-five (25) compounds but there was complete degradation of 13 compounds by strain YSA-7 and 17 compounds by strain YSA-8 using GC-MS. Both strains (YSA-7 and YSA8) were significant and efficient for removing Cu and Zn using mechanism of biosorption at different pH, metal concentrations and time of incubation. Remediation of hydrocarbons and metals under in vitro conditions suggests that the inoculation of such bacteria to petroleum contaminated soil possessing mixture of contaminants such as hydrocarbons and metals can remove such pollutants from contaminated environment and can restore such habitat to its original state.

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烃类利用菌和耐金属菌同时降解石油污染土壤中的烃类和脱毒金属

摘要石油对我们的生物圈，特别是土壤和水的污染是由于石化工业释放的石油产品造成的，现在是人类面临的最大问题。目前的工作是评估利用碳氢化合物和耐金属细菌在被石油污染的土壤中降解碳氢化合物和修复金属的能力，目的是形成超级生物接种剂，使土壤恢复原状。为了达到上述目的，利用气相色谱-质谱联用技术对石油污染土壤中的碳氢化合物进行了观测，在石油污染土壤中共发现25种碳氢化合物，碳氢化合物浓度为3738 mg/kg。石油污染土壤的细菌总数为2.10 × 106 CFU/g土壤。菌株YSA-7和YSA-8表现出最高的碳氢化合物利用潜力，吸光度分别为0.681和0.614。菌株对Cu和Zn也具有高度耐受性，耐受性为600 µg/ml（Cu）和1500 菌株YSA-7和700的µg/ml（Zn） µg/ml（Cu）和1800（Zn）µg/ml。菌株YSA-7和YSA-8分别经16SrRNA鉴定为梭形赖氨菌和嗜麦芽窄食单胞菌。20之后培养几天后，梭形赖氨菌菌株YSA-7和嗜麦芽窄食单胞菌YSA8降解了所有二十五（25）种化合物，但使用GC-MS，菌株YSA-8完全降解了13种化合物和17种化合物。两个菌株（YSA-7和YSA8）在不同pH、金属浓度和培养时间下利用生物吸附机制对Cu和Zn的去除都是显著有效的。在体外条件下对碳氢化合物和金属的修复表明，将这种细菌接种到含有碳氢化合物和重金属等污染物混合物的石油污染土壤中，可以从受污染的环境中去除这种污染物，并可以将这种栖息地恢复到其原始状态。

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来源期刊

Geomicrobiology Journal 环境科学-地球科学综合

CiteScore

4.80

自引率

8.70%

发文量

审稿时长

3.3 months

期刊介绍： Geomicrobiology Journal is a unified vehicle for research and review articles in geomicrobiology and microbial biogeochemistry. One or two special issues devoted to specific geomicrobiological topics are published each year. General articles deal with microbial transformations of geologically important minerals and elements, including those that occur in marine and freshwater environments, soils, mineral deposits and rock formations, and the environmental biogeochemical impact of these transformations. In this context, the functions of Bacteria and Archaea, yeasts, filamentous fungi, micro-algae, protists, and their viruses as geochemical agents are examined. Articles may stress the nature of specific geologically important microorganisms and their activities, or the environmental and geological consequences of geomicrobiological activity. The Journal covers an array of topics such as: microbial weathering; microbial roles in the formation and degradation of specific minerals; mineralization of organic matter; petroleum microbiology; subsurface microbiology; biofilm form and function, and other interfacial phenomena of geological importance; biogeochemical cycling of elements; isotopic fractionation; paleomicrobiology. Applied topics such as bioleaching microbiology, geomicrobiological prospecting, and groundwater pollution microbiology are addressed. New methods and techniques applied in geomicrobiological studies are also considered.