A. H. Rojo-Cebreros, L. Ibarra-Castro, Ernesto Guerrero-Carlock, J. L. Sánchez-Téllez, L. Álvarez-Lajonchère
{"title":"Pilot-scale production of the rotifer Brachionus sp. under different culture systems","authors":"A. H. Rojo-Cebreros, L. Ibarra-Castro, Ernesto Guerrero-Carlock, J. L. Sánchez-Téllez, L. Álvarez-Lajonchère","doi":"10.4067/S0718-19572017000300011","DOIUrl":null,"url":null,"abstract":"espanolPequenos Brachionus sp. (130-230 µm) en 2011-2012 y superpequenos Brachionus sp. (110-140 µm) en 2013-2014, se cultivaron en un sistema por lotes (ciclos de 3 o 4 dias) y en sistemas semi-continuos. Para la alimentacion de rotiferos se usaron microalga concentrada (Nannochloropsis sp.), dosificada continuamente con una bomba peristaltica. Las condiciones del cultivo fueron provistas con aire, oxigeno e hidroximetanosulfonato de sodio. La produccion total promedio por dia fue 552, 602 y 459 millones de rotiferos d-1, con una densidad final promedio de cosecha de 1.099 rotiferos mL-1, 1.052 rotiferos mL-1 y 1.015 rotiferos mL-1, para tres dias (3-d), cuatro dias (4-d) del sistema de produccion por lotes y el sistema de produccion semi-continuo, respectivamente. Los valores promedios producidos de rotiferos fueron adecuados para suplir los requerimientos de rotiferos en los ciclos de cultivos piloto, para producir 169 x 10³/ano (2012) a 564 x 10³ (2013) juveniles/ano con una demanda media anual de 83,9 x 10(6) rotiferos/1000 juveniles producidos. Los costos totales operacionales por millon de rotiferos/dia fueron bajos con el sistema de cultivo semi-continuo ($0,23), seguido por el sistema por lotes de 4-d ($0,55) y el sistema por lotes de 3-d ($0,59). Estos costos de produccion fueron mas bajos que otros reportes de produccion con alimento artificial y sistemas de recirculacion. Los principales componentes de los costos operacionales fueron alimento (71-77%) y personal (7-11%). La mejor estabilidad y confiabilidad de la produccion fue con el sistema semi-continuo, el cual garantizo las cantidades requeridas diariamente para los cultivos de cria larval mas altos. Para posibles mejoras y aumento de la produccion, la discusion se basa en terminos de eficiencia financiera. EnglishSmall Brachionus sp. (130-230 µm) in 2011-2012 and tiny Brachionus sp. (110-140 µm) in 2013-2014 were reared in batch culture (3 or 4 day cycles) and semi-continuous systems. For feeding rotifers, nonviable microalgae (Nannochloropsis sp.) were used and were continuously dosed with peristaltic pumps. The cultures provided air, oxygen, and sodium hydroxymethanesulfonate. An average total daily production of 552, 602 and 459 million rotifers d-1 with mean final densities of 1,099 rotifers mL-1, 1,052 rotifers mL-1 and 1,015 rotifers mL-1 were harvested in three day (3-d) and four day (4-d) batch culture systems and semi-continuous culture systems, respectively. The average values of rotifers produced were adequate to supply the rotifers required in the parallel pilot rearing larval cycles, and 169 x 10³/yr (2012) to 564 x 10³ (2013) juveniles/yr were produced with a mean annual demand of 83.9 x 10(6) rotifers/1,000 juveniles produced. The total operational cost per million rotifers/day was lower for the semi-continuous culture system ($0.23), followed by the 4-d batch system ($0.55) and 3-d batch system ($0.59). These production costs were lower than those of other reports with artificial feeds and recirculation systems. The main components of the total operational cost was food (71-77%) and labor (7-11%). The best production stability and reliability were in the semi-continuous system, which best met the required daily quantities for the larval rearing trials. For possible improvements and increased production, the results are discussed in terms of financial efficiency.","PeriodicalId":0,"journal":{"name":"","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2017-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.4067/S0718-19572017000300011","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.4067/S0718-19572017000300011","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2
Abstract
espanolPequenos Brachionus sp. (130-230 µm) en 2011-2012 y superpequenos Brachionus sp. (110-140 µm) en 2013-2014, se cultivaron en un sistema por lotes (ciclos de 3 o 4 dias) y en sistemas semi-continuos. Para la alimentacion de rotiferos se usaron microalga concentrada (Nannochloropsis sp.), dosificada continuamente con una bomba peristaltica. Las condiciones del cultivo fueron provistas con aire, oxigeno e hidroximetanosulfonato de sodio. La produccion total promedio por dia fue 552, 602 y 459 millones de rotiferos d-1, con una densidad final promedio de cosecha de 1.099 rotiferos mL-1, 1.052 rotiferos mL-1 y 1.015 rotiferos mL-1, para tres dias (3-d), cuatro dias (4-d) del sistema de produccion por lotes y el sistema de produccion semi-continuo, respectivamente. Los valores promedios producidos de rotiferos fueron adecuados para suplir los requerimientos de rotiferos en los ciclos de cultivos piloto, para producir 169 x 10³/ano (2012) a 564 x 10³ (2013) juveniles/ano con una demanda media anual de 83,9 x 10(6) rotiferos/1000 juveniles producidos. Los costos totales operacionales por millon de rotiferos/dia fueron bajos con el sistema de cultivo semi-continuo ($0,23), seguido por el sistema por lotes de 4-d ($0,55) y el sistema por lotes de 3-d ($0,59). Estos costos de produccion fueron mas bajos que otros reportes de produccion con alimento artificial y sistemas de recirculacion. Los principales componentes de los costos operacionales fueron alimento (71-77%) y personal (7-11%). La mejor estabilidad y confiabilidad de la produccion fue con el sistema semi-continuo, el cual garantizo las cantidades requeridas diariamente para los cultivos de cria larval mas altos. Para posibles mejoras y aumento de la produccion, la discusion se basa en terminos de eficiencia financiera. EnglishSmall Brachionus sp. (130-230 µm) in 2011-2012 and tiny Brachionus sp. (110-140 µm) in 2013-2014 were reared in batch culture (3 or 4 day cycles) and semi-continuous systems. For feeding rotifers, nonviable microalgae (Nannochloropsis sp.) were used and were continuously dosed with peristaltic pumps. The cultures provided air, oxygen, and sodium hydroxymethanesulfonate. An average total daily production of 552, 602 and 459 million rotifers d-1 with mean final densities of 1,099 rotifers mL-1, 1,052 rotifers mL-1 and 1,015 rotifers mL-1 were harvested in three day (3-d) and four day (4-d) batch culture systems and semi-continuous culture systems, respectively. The average values of rotifers produced were adequate to supply the rotifers required in the parallel pilot rearing larval cycles, and 169 x 10³/yr (2012) to 564 x 10³ (2013) juveniles/yr were produced with a mean annual demand of 83.9 x 10(6) rotifers/1,000 juveniles produced. The total operational cost per million rotifers/day was lower for the semi-continuous culture system ($0.23), followed by the 4-d batch system ($0.55) and 3-d batch system ($0.59). These production costs were lower than those of other reports with artificial feeds and recirculation systems. The main components of the total operational cost was food (71-77%) and labor (7-11%). The best production stability and reliability were in the semi-continuous system, which best met the required daily quantities for the larval rearing trials. For possible improvements and increased production, the results are discussed in terms of financial efficiency.