Comparison of Antibiotic Susceptibility of Carbapanem-Resistant Enterobactericeae Species with Two Different Panels (Phoenix BD)

Hacer Uğurlu, Burak Küçük, F. Orak, M. Aral
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Abstract

Objective: The aim of this study; The aim of this study is to compare the antibiotic susceptibility of carbapanem-resistant Enterobactericeae strains isolated in the microbiology laboratory of our hospital with two different panels and to evaluate the performance of the CPO test. Material & Method: The samples sent to the microbiology laboratory of our hospital between 01.01.2020 - 31.12.2020 were evaluated. Specimens with growth were loaded onto the BD Phoenix 100 automated system BD Phoenix NMIC 433 panel, and 50 isolates of Enterobacteriaceae that resulted in carbapenem resistant were studied with the BD Phoenix NMIC 505 CPO panel. 50 carbapenem-resistant Enterobacteriaceae isolates were classified according to Ambler class A, B, and D by the CPO panel. Results: Of the isolates, 28 (56%) were isolated from male patients and 22 (44%) from female patients. Of the carbapenem-resistant Enterobacteriaceae isolates, 43 (86%) were identified as Klebsiella pneumoniae and 4 (8%) were Escherichia coli. When the evaluation of antibiotic susceptibility rates of the CPO panel and NMIC 433 panel of carbapenem-resistant Enterobacteriaceae isolates were examined; Antibiotic susceptibility results of 50 Enterobacteriaceae isolates studied in both panels were found to be the same. When the antibiotics that can be studied in the CPO panel were examined, it was observed that the most effective antibiotic was ceftazidime-avibactam. When the results were examined, it was observed that 46 (92%) isolates were susceptible to ceftazidime-avibactam. The number of bacteria producing class B carbapenemase was 4 (8%) and the number of bacteria producing class D carbapenemase was 42 (84%). Conclusion: It should be noted that ceftazidime avibactam can be used as a treatment option in carbapenem-resistant species. Further work on the CPO panel will help identify the contribution of the CPO panel to the routine workflow.
两种不同菌株(Phoenix BD)对Carapanem耐药肠杆菌的耐药性比较
目的:本研究的目的;本研究的目的是比较在我院微生物实验室分离的耐卡巴烯肠杆菌科菌株与两个不同小组的抗生素敏感性,并评估CPO测试的性能。材料与方法:对2020年1月1日至2020年12月31日期间送往我院微生物实验室的样本进行评估。将生长的样品加载到BD Phoenix 100自动化系统BD PhoenixNMIC 433板上,并用BD Phoenix-NMIC 505 CPO板研究导致碳青霉烯耐药性的50个肠杆菌科分离株。CPO小组根据Ambler A级、B级和D级对50株耐碳青霉烯类肠杆菌科分离株进行了分类。结果:28株(56%)分离自男性患者,22株(44%)分离自女性患者。在耐碳青霉烯的肠杆菌科分离株中,43株(86%)被鉴定为肺炎克雷伯菌,4株(8%)为大肠杆菌。当对耐碳青霉烯类肠杆菌科分离株的CPO小组和NMIC 433小组的抗生素敏感率进行评估时;在两个小组中研究的50个肠杆菌科分离株的抗生素敏感性结果是相同的。当检查CPO小组中可以研究的抗生素时,发现最有效的抗生素是头孢他啶-阿维巴坦。当检查结果时,观察到46个(92%)分离株对头孢他啶-阿维巴坦敏感。产生B类碳青霉烯酶的细菌数量为4(8%),产生D类碳青霉烷酶的细菌数目为42(84%)。结论:值得注意的是,头孢他啶-阿维巴坦可作为碳青霉烯类耐药菌株的治疗选择。CPO面板的进一步工作将有助于确定CPO面板对日常工作流程的贡献。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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