Study of gene correlation between endothelial growth factor family in mouse eye

Q4 Medicine

中华眼底病杂志 Pub Date : 2020-03-25 DOI:10.3760/CMA.J.CN511434-20180803-00269

Jinglin Cui, Hang Lu, Jingyu Chen, Binjiang Luo, Weiwei Xiu, Huimei Li, Jiao-Ying Huang, Yuhan Chen, Jingyu Chen

{"title":"Study of gene correlation between endothelial growth factor family in mouse eye","authors":"Jinglin Cui, Hang Lu, Jingyu Chen, Binjiang Luo, Weiwei Xiu, Huimei Li, Jiao-Ying Huang, Yuhan Chen, Jingyu Chen","doi":"10.3760/CMA.J.CN511434-20180803-00269","DOIUrl":null,"url":null,"abstract":"Objective \nTo compare the function and action pathways of VEGFA, VEGFB and VEGFC in VEGF family of mouse eye. \n \n \nMethods \nUsing the BXD mouse gene data in Genenetwork database as template to compare and study the similarities and differences of VEGFA, VEGFB and VEGFC molecular pathways or potential functions in the whole genome expression spectrum of BXD recombinant mouse inbred line population, with multiple analytical methods and statistical strategies were used, such as gene expression level, target genes comparison, top genes comparison associated to target genes, expression Quantitative Trait Loci (eQTL). \n \n \nResults \nMatrix comparison showed strong positive correlation between two probes of VEGFC (r=0.732, P 0.05). In the comparative analysis of the relevant Top50 genes of each VEGF gene, most of the genes in BXD mouse were not significantly correlated with VEGFA, VEGFB and VEGFC except for the weak association of individual related genes. The results of eQTL analysis showed that each probe of VEGF gene was located on different chromosomes. \n \n \nConclusions \nThe expression levels and positive and negative correlations of VEGFA, VEGFB and VEGFC were different in the VEGF family of mouse eye, suggesting that these genes may play their role through different pathways. \n \n \nKey words: \nVascular endothelial growth factors; DNA probes; Genome components","PeriodicalId":10103,"journal":{"name":"中华眼底病杂志","volume":"36 1","pages":"211-219"},"PeriodicalIF":0.0000,"publicationDate":"2020-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中华眼底病杂志","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3760/CMA.J.CN511434-20180803-00269","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Objective To compare the function and action pathways of VEGFA, VEGFB and VEGFC in VEGF family of mouse eye. Methods Using the BXD mouse gene data in Genenetwork database as template to compare and study the similarities and differences of VEGFA, VEGFB and VEGFC molecular pathways or potential functions in the whole genome expression spectrum of BXD recombinant mouse inbred line population, with multiple analytical methods and statistical strategies were used, such as gene expression level, target genes comparison, top genes comparison associated to target genes, expression Quantitative Trait Loci (eQTL). Results Matrix comparison showed strong positive correlation between two probes of VEGFC (r=0.732, P 0.05). In the comparative analysis of the relevant Top50 genes of each VEGF gene, most of the genes in BXD mouse were not significantly correlated with VEGFA, VEGFB and VEGFC except for the weak association of individual related genes. The results of eQTL analysis showed that each probe of VEGF gene was located on different chromosomes. Conclusions The expression levels and positive and negative correlations of VEGFA, VEGFB and VEGFC were different in the VEGF family of mouse eye, suggesting that these genes may play their role through different pathways. Key words: Vascular endothelial growth factors; DNA probes; Genome components

查看原文本刊更多论文

小鼠眼内皮生长因子家族基因相关性研究

目的比较小鼠眼VEGF家族中VEGFA、VEGFB和VEGFC的功能及作用途径。方法以genennetwork数据库中的BXD小鼠基因数据为模板，采用基因表达水平、靶基因比较、靶基因相关顶级基因比较等多种分析方法和统计策略，比较研究BXD重组小鼠自交系群体全基因组表达谱中VEGFA、VEGFB和VEGFC分子通路或潜在功能的异同。表达数量性状位点(eQTL)。结果两种探针间VEGFC呈显著正相关(r=0.732, p0.05)。在对各VEGF基因相关Top50基因的对比分析中，BXD小鼠除个别相关基因存在弱相关性外，大部分基因与VEGFA、VEGFB、VEGFC均无显著相关性。eQTL分析结果显示，VEGF基因的每个探针位于不同的染色体上。结论VEGFA、VEGFB和VEGFC在小鼠眼VEGF家族中的表达水平及正、负相关存在差异，提示这些基因可能通过不同的途径发挥作用。关键词:血管内皮生长因子;DNA探针;基因组的组件

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

中华眼底病杂志 Medicine-Ophthalmology

CiteScore

0.40

自引率

0.00%

发文量

5383

期刊介绍： Chinese Journal of Ocular Fundus Diseases is the only scientific journal in my country that focuses on reporting fundus diseases. Its purpose is to combine clinical and basic research, and to give equal importance to improvement and popularization. It comprehensively reflects the leading clinical and basic research results of fundus disease disciplines in my country; cultivates professional talents in fundus disease, promotes the development of fundus disease disciplines in my country; and promotes academic exchanges on fundus disease at home and abroad. The coverage includes clinical and basic research results of posterior segment diseases such as retina, uveal tract, vitreous body, visual pathway, and internal eye diseases related to systemic diseases. The readers are medical workers and researchers related to clinical and basic research of fundus diseases. According to the journal retrieval report of the Chinese Institute of Scientific and Technological Information, the comprehensive ranking impact factor and total citation frequency of the Chinese Journal of Ocular Fundus Diseases have been among the best in the disciplines of ophthalmology, otolaryngology, and ophthalmology in my country for many years. The papers published have been included in many important databases at home and abroad, such as Scopus, Peking University Core, and China Science Citation Database (CSCD).