An integrative study of morphological and molecular characterization of Diplectanum aequans (Diplectanidae: Monogenea) infecting European sea bass Dicentrarchus labrax (L.) from Turkish coasts

IF 0.7 4区农林科学 Q3 VETERINARY SCIENCES

Ankara Universitesi Veteriner Fakultesi Dergisi Pub Date : 2022-02-15 DOI:10.33988/auvfd.987702

C. Aydın, G. Z. Pekmezci

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引用次数: 1

Abstract

The diplectanid monogenean Diplectanum aequans ( D. aequans ) (Wegener, 1857) is one of the most important ectoparasites in the wild and cultured European sea bass Dicentrarchus labrax ( Dic. labrax ) (Linnaeus, 1758) worldwide. Presently, there is no information on the molecular data of D. aequans from Turkish coasts. In the present study, diplectanid monogeneans were collected from wild and cultured Dic. labrax in the Black Sea (FAO area 37.4.2) and Aegean Sea (FAO area 37.1.3) coasts of Türkiye, morphologically identified, and genetically characterized by sequencing of 28S rRNA and 18S rRNA fragments. The collected diplectanids were unambiguously identified as D. aequans based on detailed morphological features and sequence characterization of partial 28S rRNA and 18S rRNA genes. The overall prevalence and mean intensity of D. aequans were 100% and 15.1, respectively. This study reports first information of molecular (28S and 18S rRNA) evidence of D. aequans from Dic. labrax in the Turkish coasts. The new 18S and 28S rRNA sequences for D. aequans isolated from the Black Sea and Aegean Sea are genetically characterized. The 28S and 18S rRNA sequences of D. aequans can be used to resolve the phylogenetic positions of species found in the family Diplectanidae from the Black and Mediterranean Sea.

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土耳其海岸欧洲黑鲈感染水双斑绦虫(双斑绦虫科:单属)形态和分子特征的综合研究

双尾目单尾目双尾目（D.aequans）（Wegener，1857）是世界各地野生和养殖的欧洲鲈鱼Dicentrarchus labrax（Dic.labrax）（Linnaeus，1758）中最重要的体外寄生虫之一。目前，还没有关于土耳其海岸的D.aequans分子数据的信息。在本研究中，从野生和培养的Dic中采集了双锥虫和单烯虫。土耳其黑海（粮农组织区域37.4.2）和爱琴海（粮农组织地区37.1.3）海岸的labrax，通过28S rRNA和18S rRNA片段测序进行形态鉴定和基因特征鉴定。根据部分28S rRNA和18S rRNA基因的详细形态特征和序列特征，收集到的二聚体被明确鉴定为D.aequans。伊豆的总患病率和平均强度分别为100%和15.1。本研究报道了来自Dic的D.aequans的分子（28S和18S rRNA）证据的首次信息。土耳其海岸的labrax。对从黑海和爱琴海分离得到的伊豆新的18S和28S rRNA序列进行了遗传学表征。阿氏锥虫的28S和18S rRNA序列可用于解析在黑海和地中海的双锥虫科中发现的物种的系统发育位置。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ankara Universitesi Veteriner Fakultesi Dergisi 农林科学-兽医学

CiteScore

1.50

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： Ankara Üniversitesi Veteriner Fakültesi Dergisi is one of the journals’ of Ankara University, which is the first well-established university in the Republic of Turkey. Research articles, short communications, case reports, letter to editor and invited review articles are published on all aspects of veterinary medicine and animal science. The journal is published on a quarterly since 1954 and indexing in Science Citation Index-Expanded (SCI-Exp) since April 2007.