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Effect of ionic strength on the interaction of AuNPs with calf spleen DNA

IF 2.1 4区工程技术 Q3 CHEMISTRY, INORGANIC & NUCLEAR

Gold Bulletin Pub Date : 2022-12-22 DOI:10.1007/s13404-022-00322-y

Monira M. Rageh, M. H. Gaber, Samar M. Mostafa

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Abstract

Abstract

Gold nanoparticles (AuNPs) are well-known biomedical and biotechnological applications because of their interesting properties. They easily cross the cell membranes and interact with intracellular materials. This study was designed to investigate the interaction of calf spleen DNA with AuNPs at a molar ratio of 2:1 in an aqueous solution with different ionic strengths (10, 50, and 100%). AuNPs and AuNPs/DNA complex were characterized by different techniques such as UV/Vis spectrophotometry, transmission electron microscopy (TEM), dynamic light scattering (DLS), and Fourier transform IR spectrophotometry. The results revealed that the maximum absorption (λ_max) of AuNPs synthesis was observed at 520 nm, and the average particle size was about 13 nm. In addition to a negative zeta potential (− 37 mV), the interaction of AuNPs with DNA was confirmed by melting point and TEM. The melting point that reflects the DNA became unstable in the presence of AuNPs, and the melting temperature decreased by about 3–5 °C with different ionic strength. Additionally, the TEM image of AuNPs/DNA complex obviously illustrated the location of AuNPs on the DNA groove. Finally, these results clearly indicate the attachment of AuNPs with DNA.

Abstract Image

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离子强度对AuNPs与小牛脾脏DNA相互作用的影响

摘要纳米金因其独特的性质在生物医学和生物技术领域有着广泛的应用。它们很容易穿过细胞膜并与细胞内物质相互作用。本研究旨在研究小牛脾脏DNA与AuNPs在不同离子强度(10%、50%和100%)水溶液中摩尔比为2:1的相互作用。采用紫外/可见分光光度法、透射电镜(TEM)、动态光散射(DLS)和傅立叶变换红外分光光度法等技术对AuNPs和AuNPs/DNA复合物进行了表征。结果表明，合成AuNPs的最大吸收峰(λmax)为520 nm，平均粒径约为13 nm;除了负zeta电位(- 37 mV)外，通过熔点和透射电镜证实了AuNPs与DNA的相互作用。反映DNA的熔点在AuNPs存在下变得不稳定，不同离子强度下的熔点温度降低约3-5℃。此外，AuNPs/DNA复合物的TEM图像清楚地显示了AuNPs在DNA凹槽上的位置。最后，这些结果清楚地表明AuNPs与DNA的附着。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Gold Bulletin

Gold Bulletin Chemistry-Inorganic Chemistry

CiteScore

3.70

自引率

4.50%

发文量

21

期刊介绍： Gold Bulletin is the premier international peer reviewed journal on the latest science, technology and applications of gold. It includes papers on the latest research advances, state-of-the-art reviews, conference reports, book reviews and highlights of patents and scientific literature. Gold Bulletin does not publish manuscripts covering the snthesis of Gold nanoparticles in the presence of plant extracts or other nature-derived extracts. Gold Bulletin has been published over 40 years as a multidisciplinary journal read by chemists, physicists, engineers, metallurgists, materials scientists, biotechnologists, surface scientists, and nanotechnologists amongst others, both within industry and academia. Gold Bulletin is published in Association with the World Gold Council.

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