{"title":"Kinetic Evaluation of the Inhibition of Acetylcholinesterase for use as a biosensor","authors":"R. Bhuvanagayathri, D. K. Daniel, G. Nirmala","doi":"10.22146/ajche.56709","DOIUrl":null,"url":null,"abstract":"The release of pesticides into the environment has increased , and there is a lack of monitoring of these contaminants. Since the conventional methods of monitoring these contaminants are compl icated , costly and time - consuming, mechanisms based on ace t ylcholinesterase inhibition have emerged as simple and rapid tools for such applications. However, the acetylcholinesterase ’s effectiveness as a sensing element in such biosensor systems depend s on the conditions selected to measure acetylcholinesterase activity and the concentration of substrate or inhibitor, which in turn affect the reaction rates. Therefore, i n the present work, the factors affecting the acetylcholinesterase activity were investigated and inhibition experiments were carried out to evaluate the kinetic parameters. The inhibition rate constant for acetylcholinesterase K i was found to be 1.9 ppm. The kinetic parameter K m was found to be 3.8mM and V max was found to be 1.3µM/min from the Eadie-Hofstee plot. The kinetic study using Lineweaver-Burk method showed mixed type of inhibition of acetylcholinesterase with carbofuran.","PeriodicalId":8490,"journal":{"name":"ASEAN Journal of Chemical Engineering","volume":"20 1","pages":"99-108"},"PeriodicalIF":0.0000,"publicationDate":"2020-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"ASEAN Journal of Chemical Engineering","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.22146/ajche.56709","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"Chemical Engineering","Score":null,"Total":0}
引用次数: 0
Abstract
The release of pesticides into the environment has increased , and there is a lack of monitoring of these contaminants. Since the conventional methods of monitoring these contaminants are compl icated , costly and time - consuming, mechanisms based on ace t ylcholinesterase inhibition have emerged as simple and rapid tools for such applications. However, the acetylcholinesterase ’s effectiveness as a sensing element in such biosensor systems depend s on the conditions selected to measure acetylcholinesterase activity and the concentration of substrate or inhibitor, which in turn affect the reaction rates. Therefore, i n the present work, the factors affecting the acetylcholinesterase activity were investigated and inhibition experiments were carried out to evaluate the kinetic parameters. The inhibition rate constant for acetylcholinesterase K i was found to be 1.9 ppm. The kinetic parameter K m was found to be 3.8mM and V max was found to be 1.3µM/min from the Eadie-Hofstee plot. The kinetic study using Lineweaver-Burk method showed mixed type of inhibition of acetylcholinesterase with carbofuran.