Pharmacoligical characterization of the iranian Cerastes cerastes gasperettii (Reptilia: Ophidia: Viperidae) venom

Q3 Nursing
S. Zaeri, Z. Aghaei, N. Mashayekhi, A. Salemi, R. Seyedian
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引用次数: 1

Abstract

Objective: Snake envenomation is common in tropical and subtropical countries of the Middle East areas including Iran. Cerastes cerastes gasperettii is a dangerous snake living in southwestern provinces of Iran. It causes massive edema at the bite site and coagulopathy leading to death if untreated. Methods: The purpose of this preliminary animal study was to evaluate the toxicity and proteomic of this venom for the first time in Iran. Moreover, the hemodynamic changes with intravenous injection of the venom were assessed and inotropic in addition to arrhythmogenic properties of this venom were investigated. Results: The estimated amount of the LD50 with intraperitoneal injection was slightly less than the similar experiment in Saudi Arabia (1.32 mg/kg versus 978 µg/kg body weight). There were 8 distinct protein bands between 12 and 66 kDa in SDS-PAGE analysis that were different with Moroccan experiment due to inter and intra species variation. Inotropic potencies were not significant since the lethal dose with intravenous injection was much lower than the Arabian experiment in guinea pigs (2.4 mg/kg versus 0.8 mg/kg). Conclusion: According to the low hemodynamic changes induced with the venom, it seems that coagulopathy and edema are the most dangerous effects of this rare snake in Iran.
伊朗蜡蚧(爬行纲:蛇亚目:蝰蛇科)毒液的药理学特征
目的:蛇中毒在包括伊朗在内的中东地区的热带和亚热带国家很常见。cerecastgasperettii是一种生活在伊朗西南部省份的危险蛇。如果不治疗,它会在咬伤部位引起大面积水肿和凝血功能障碍,导致死亡。方法:首次在伊朗对该毒液的毒性和蛋白质组学进行初步的动物研究。此外,我们还评估了静脉注射该毒液后血液动力学的变化,并研究了该毒液的肌力和致心律失常特性。结果:腹腔注射LD50的估计量略低于沙特阿拉伯的类似实验(1.32 mg/kg vs 978µg/kg体重)。由于种内和种间的差异,SDS-PAGE分析结果显示在12 ~ 66 kDa之间有8条不同的蛋白带,与摩洛哥实验结果不同。由于静脉注射的致死剂量远低于阿拉伯实验中的豚鼠(2.4 mg/kg对0.8 mg/kg),因此肌力的效力并不显著。结论:根据该毒液引起的低血流动力学变化,凝血功能障碍和水肿似乎是该伊朗稀有蛇最危险的影响。
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来源期刊
Journal of Emergency Practice and Trauma
Journal of Emergency Practice and Trauma Nursing-Emergency Nursing
CiteScore
0.50
自引率
0.00%
发文量
13
审稿时长
12 weeks
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