ACCURATE DETECTION OF VIRAL SEROTYPE DENGUE HEMORRHAGIC FEVER THROUGH
AEDES SP MOSQUITOES USING REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION
(RT-PCR)
Erlin Yustin Tatontos, Fihiruddin Fihiruddin, N. Inayati
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引用次数: 0
Abstract
Dengue Hemorrhagic Fever (DHF) caused Dengue virus which transmitted
through the bite of the Aedes sp. Detection of dengue virus serotypes is
essential for epidemiology as well as potential disease pathogens. RT-PCR
method was more effective in mosquitoes, because the virus contained in the
mosquito body is passed on to the next generation (trans ovarian). The
purpose of this research is to know the serotype of DHF virus accurately
through vector mosquito using RT-PCR method in Mataram City, so that vector
control, early diagnosis and management of DHF disease could be done quickly
and precisely. This research was a laboratory explorative research with
cross sectional design that determines serotype of DHF virus through vector
mosquito using RT-PCR method in Mataram City. The population of this study
were Aedes aegypti female mosquitoes from eggs. Collected from
houses located in Community Health Center Mataram city worked area with high
dengue cases: Pagesangan, Karang Taliwang, Karang Pule, Tanjung Karang,
Mataram, and Selaparang especially around the house’s sufferers. The results
showed that only the mosquito group from the worked area of the Mataram
Community Health Center showed the ribbon image with the base pair in
accordance with serotype 1 (DENGUE 1), the other negative.