Heterogeneity of Yersinia ruckeri Isolated from Rainbow Trout (Oncorhynchus mykiss) Infected with Enteric Red Mouth Disease

Q4 Veterinary

World''s Veterinary Journal Pub Date : 2022-01-25 DOI:10.54203/scil.2022.wvj3

F. Aflakian, Helia Nikoueian, H. Salimizand, M. Rad, A. R. Zomorodi

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引用次数: 0

Abstract

Regarding the increasing prevalence of antimicrobial resistance as a global menace, typing procedures are of great importance in epidemiological surveys. In the current study, interspecies differences of Yersinia ruckeri (Y. ruckeri) isolates were detected by antimicrobial susceptibility profiling and molecular traits to determine the relationship between isolates. The current study was conducted on 27 Y. ruckeri isolates collected from 40 infected rainbow trout (Oncorhynchus mykiss) with clinical enteric red mouth disease in Mashhad, Iran. Interspecies differences of all isolates were detected by antimicrobial susceptibility profiling using disk diffusion method, Repetitive Extragenic Palindromic PCR (REP-PCR), Enterobacterial Repetitive Intergenic Consensus PCR (ERIC-PCR), and Random Amplification of Polymorphic DNA PCR (RAPD-PCR) to determine the relationship between isolates. Simpson’s diversity index was calculated for each typing technique. The most phenotype resistant was against ampicillin, chloramphenicol, and lincomycin. Furthermore, enrofloxacin and ciprofloxacin showed the highest activity (100%) against isolates. According to the results, the highest diversity index was observed in the REP-PCR method (D = 0.91). In conclusion, REP-PCR can be a powerful technique for epidemiological studies.

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虹鳟感染肠道红口病的拉克氏耶尔森菌的异质性

鉴于抗微生物耐药性的日益流行是一种全球威胁，分型程序在流行病学调查中具有重要意义。在本研究中，通过对鲁氏耶尔森菌（Y.ruckeri）分离株的耐药性分析和分子特征来检测其种间差异，以确定分离株之间的关系。目前的研究是对从伊朗马什哈德市40条患有临床肠道红口病的虹鳟（Oncorhynchus mykiss）身上采集的27株布鲁克里氏杆菌分离株进行的。采用纸片扩散法、重复基因外回文PCR（REP-PCR）、肠道细菌重复基因间一致性PCR（ERIC-PCR）和随机扩增多态性DNA PCR（RAPD-PCR）进行抗菌药物敏感性分析，检测所有分离株的种间差异，以确定分离株之间的关系。针对每种打字技术计算Simpson多样性指数。对氨苄青霉素、氯霉素和林可霉素的表型抗性最强。此外，恩诺沙星和环丙沙星对分离株的活性最高（100%）。结果表明，REP-PCR方法的多样性指数最高（D=0.91）。总之，REP-PCR可以作为一种强有力的流行病学研究技术。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

World''s Veterinary Journal Veterinary-Veterinary (all)

CiteScore

1.00

自引率

0.00%

发文量

期刊介绍： The World''s Veterinary Journal (ISSN 2322-4568) is an international, peer reviewed open access journal aims to publish the high quality material from veterinary scientists'' studies. All accepted articles are published Quarterly in full text on the Internet. WVJ publishes the results of original scientific researches, reviews, case reports and short communications, in all fields of veterinary science. In details, topics are: Behavior Environment and welfare Animal reproduction and production Parasitology Endocrinology Microbiology Immunology Pathology Pharmacology Epidemiology Molecular biology Immunogenetics Surgery Virology Physiology Vaccination Gynecology Exotic animals Animal diseases Radiology Ophthalmology Dermatology Chronic disease Anatomy Non-surgical pathology issues of small to large animals Cardiology and oncology.