Introducing a bionanocomposite (Ultrasound-assisted synthesis of Ag nanoparticles embedded Aloe vera gel) for the treatment of cervical carcinoma

IF 2.6 4区材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY

Journal of Experimental Nanoscience Pub Date : 2022-11-02 DOI:10.1080/17458080.2022.2134562

Jing Yan, B. Karmakar, M. S. A. Zaki, R. Osman, A. Abdalla, A. Shati, A. El‐kott, Fatimah M. Yousef, S. S. Aggad, G. Batiha

{"title":"Introducing a bionanocomposite (Ultrasound-assisted synthesis of Ag nanoparticles embedded Aloe vera gel) for the treatment of cervical carcinoma","authors":"Jing Yan, B. Karmakar, M. S. A. Zaki, R. Osman, A. Abdalla, A. Shati, A. El‐kott, Fatimah M. Yousef, S. S. Aggad, G. Batiha","doi":"10.1080/17458080.2022.2134562","DOIUrl":null,"url":null,"abstract":"Abstract Regarding applied, facile, green chemical research, a bio-inspired approach is being reported for the sonochemical synthesis of Ag nanoparticles by Aloe vera gel extract as a natural reducing/stabilising and solid support agent without using any toxic and harmful reagent. The biosynthesized Ag NPs/A. vera gel bionanocomposite was characterised by advanced physicochemical techniques like ultraviolet-visible (UV–Vis), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), X-ray Diffraction (XRD) and elemental mapping study. It has been established that Aloe vera gel-stabilized silver nanoparticles have a spherical shape with a mean diameter of around 35–40 nm. To investigate the antioxidant potentials of Ag NPs/A. vera gel, one of the common antioxidant techniques i.e. DPPH was used. The results of DPPH test proved the excellent antioxidant properties of Ag NPs/A. vera gel in comparison to the positive control used i.e. butylated hydroxytoluene. The IC50 of Ag NPs/A. vera gel was 42 µg/mL in the antioxidant test. Thereafter, on having a significant IC50 value in radical scavenging assay, we extended the bio-application of the desired nanocomposite in the anticancer study. To survey the cytotoxicity and anti-human cervical carcinoma effects of Ag NPs/A. vera gel, MTT assay was used on CC1-PI19, SiHa, HeLa and HNCF-PI52 cell lines. The IC50 of the Ag NPs/A. vera gel was 16, 13, 15 and 11 µg/mL against CC1-PI19, SiHa, HeLa and HNCF-PI52 cell lines, respectively. As mentioned, the Ag NPs/A. vera gel had significant antioxidant and anti-human cervical cancer effects. After approving the above results in the clinical trial studies, the Ag NPs/A. vera gel can be used as a chemotherapeutic drug for the treatment of several types of cervical cancers. According to the above results, the significant anti-cervical cancer effects of Ag NPs/A. vera gel are confirmed.","PeriodicalId":15673,"journal":{"name":"Journal of Experimental Nanoscience","volume":"17 1","pages":"617 - 630"},"PeriodicalIF":2.6000,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Experimental Nanoscience","FirstCategoryId":"88","ListUrlMain":"https://doi.org/10.1080/17458080.2022.2134562","RegionNum":4,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 2

Abstract

Abstract Regarding applied, facile, green chemical research, a bio-inspired approach is being reported for the sonochemical synthesis of Ag nanoparticles by Aloe vera gel extract as a natural reducing/stabilising and solid support agent without using any toxic and harmful reagent. The biosynthesized Ag NPs/A. vera gel bionanocomposite was characterised by advanced physicochemical techniques like ultraviolet-visible (UV–Vis), Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray spectroscopy (EDX), X-ray photoelectron spectroscopy (XPS), X-ray Diffraction (XRD) and elemental mapping study. It has been established that Aloe vera gel-stabilized silver nanoparticles have a spherical shape with a mean diameter of around 35–40 nm. To investigate the antioxidant potentials of Ag NPs/A. vera gel, one of the common antioxidant techniques i.e. DPPH was used. The results of DPPH test proved the excellent antioxidant properties of Ag NPs/A. vera gel in comparison to the positive control used i.e. butylated hydroxytoluene. The IC50 of Ag NPs/A. vera gel was 42 µg/mL in the antioxidant test. Thereafter, on having a significant IC50 value in radical scavenging assay, we extended the bio-application of the desired nanocomposite in the anticancer study. To survey the cytotoxicity and anti-human cervical carcinoma effects of Ag NPs/A. vera gel, MTT assay was used on CC1-PI19, SiHa, HeLa and HNCF-PI52 cell lines. The IC50 of the Ag NPs/A. vera gel was 16, 13, 15 and 11 µg/mL against CC1-PI19, SiHa, HeLa and HNCF-PI52 cell lines, respectively. As mentioned, the Ag NPs/A. vera gel had significant antioxidant and anti-human cervical cancer effects. After approving the above results in the clinical trial studies, the Ag NPs/A. vera gel can be used as a chemotherapeutic drug for the treatment of several types of cervical cancers. According to the above results, the significant anti-cervical cancer effects of Ag NPs/A. vera gel are confirmed.

查看原文本刊更多论文

介绍一种生物纳米复合材料(超声辅助合成银纳米颗粒嵌入芦荟凝胶)用于治疗宫颈癌

摘要关于应用、简单、绿色的化学研究，报道了一种生物启发的方法，通过芦荟凝胶提取物作为天然还原/稳定和固体支持剂，在不使用任何有毒有害试剂的情况下，通过声化学合成银纳米颗粒。生物合成的Ag NPs/A。采用先进的物理化学技术，如紫外-可见光谱（UV–Vis）、扫描电子显微镜（SEM）、透射电子显微镜（TEM）、能量分散X射线光谱（EDX）、X射线光电子能谱（XPS）、X衍射（XRD）和元素图谱研究，对维拉凝胶-生物纳米复合材料进行了表征。已经证实，芦荟凝胶稳定的银纳米颗粒呈球形，平均直径约为35-40 nm。研究Ag NPs/A的抗氧化能力。使用了一种常见的抗氧化剂技术，即DPPH。DPPH试验结果证明Ag NPs/A具有良好的抗氧化性能。vera凝胶与使用的阳性对照即丁基羟基甲苯相比。Ag NPs/A的IC50。维拉凝胶是42 抗氧化测试中为µg/mL。此后，由于在自由基清除测定中具有显著的IC50值，我们扩展了所需纳米复合材料在抗癌研究中的生物应用。探讨Ag NPs/A的细胞毒性和抗人宫颈癌作用。vera凝胶，MTT法检测CC1-PI19、SiHa、HeLa和HNCF-PI52细胞系。Ag NPs/A的IC50。维拉凝胶分别为16、13、15和11 µg/mL分别对抗CC1-PI19、SiHa、HeLa和HNCF-PI52细胞系。如前所述，Ag NPs/A。vera凝胶具有显著的抗氧化和抗人宫颈癌症作用。在临床试验研究中批准上述结果后，Ag NPs/A。vera凝胶可作为化疗药物用于治疗多种类型的宫颈癌。根据上述结果，Ag NPs/A具有显著的抗宫颈癌症作用。维拉凝胶得到证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Experimental Nanoscience 工程技术-材料科学：综合

CiteScore

4.10

自引率

25.00%

发文量

审稿时长

6.5 months

期刊介绍： Journal of Experimental Nanoscience, an international and multidisciplinary journal, provides a showcase for advances in the experimental sciences underlying nanotechnology and nanomaterials. The journal exists to bring together the most significant papers making original contributions to nanoscience in a range of fields including biology and biochemistry, physics, chemistry, chemical, electrical and mechanical engineering, materials, pharmaceuticals and medicine. The aim is to provide a forum in which cross fertilization between application areas, methodologies, disciplines, as well as academic and industrial researchers can take place and new developments can be encouraged.