Elevated levels of cytochrome p450scc and 3β-hydroxysteroid dehydrogenase expression in skin of acne vulgaris patients

Abstract

Background: Acne vulgaris belongs to androgen-dependent diseases where androgens cause sebaceous gland hypertrophy with a subsequent enlargement of sebum production. Aims: Determination of the CYP450scc 3-hydroxysteroid dehydrogenase expression profile in normal skin and skin of patients with acne. Material and methods: Immunohistochemical assay was carried out to evaluate cytochrome P450 side chain cleavage and 3-hydroxysteroid dehydrogenase expression in skin of patients with acne vulgaris and in normal skin. Results: Cytochrome P450 side chain cleavage expression was elevated in epidermal keratinocytes and sebocytes whereas 3-hydroxysteroid dehydrogenase levels were increased in mature sebocytes of acne vulgaris patients. Conclusions: The revealed alterations in expression of steroid-converting enzymes in acne skin may indicate not only the conversion of steroids produced by external steroidogenesis organs but elevated steroids local production as well. The fact may account both for sebaceous gland hyperplasia and inflammation sustenance in skin.