Sex-relevant genes in the embryo stage of Chinese soft-shelled turtles as revealed by RNA-Seq analysis

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-05-03 DOI:10.1163/15707563-bja10073

Xianwen Zhou, Hui Luo, D. Zeng, Yazhou Hu, Pei Wang, G. Xiong, Xiao-qing Wang

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引用次数: 0

Abstract

The Chinese soft-shelled turtle (Pelodiscus sinensis) is an economically important and rare freshwater aquaculture species in China. The male turtles have better economic benefits than females due to their faster growth speed. In order to explore the mechanism of sex differentiation and determination of P. sinensis, transcriptome analysis was carried out using embryo samples from the sex determination, sex differentiation and shelling stages, respectively. The gender types of embryos were identified by genotyping and histological analysis. In all, 277,230 and 273,859 genes were identified from embryos of male and female turtles, respectively. Cluster analysis of gene expression patterns exhibited trends for all differentially expressed genes (DEGs), which can be classified into three subclusters. In the sex determination, sex differentiation, and shelling stages 975, 43, and 300 DEGs, respectively, were identified by comparing the male and female groups. KEGG analysis was used to explore the function of the DEGs. Fgf9, foxl2, mex3b, sox7,tgfβ-3, wnt4, wnt7a, and wt1 were upregulated in ovary development stages and chfr, ampk, aldh2, dao, glt1d1, hvcn1, psmd9, srsf9 and ubc were upregulated in testis development stages, indicating that these genes play important roles in the sex development of P. sinensis. The results of this study provide preliminary insights into the sex determination and differentiation of Chinese soft-shelled turtles.

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用RNA-Seq分析中国鳖胚胎期性别相关基因

中华鳖是我国经济上重要的珍稀淡水养殖物种。雄龟由于生长速度快，经济效益比雌龟好。为了探讨中华鳖性别分化和性别鉴定的机制，分别利用性别鉴定、性别分化和脱壳阶段的胚胎样本进行了转录组分析。通过基因分型和组织学分析确定胚胎的性别类型。总共从雄性和雌性海龟的胚胎中分别鉴定出277230和273859个基因。基因表达模式的聚类分析显示，所有差异表达基因（DEG）都有趋势，可分为三个子聚类。在性别决定、性别分化和脱壳阶段，通过比较雄性和雌性，分别鉴定出975、43和300个DEG。KEGG分析用于探索DEG的功能。Fgf9、foxl2、mex3b、sox7、tgf-β-3、wnt4、wnt7a和wt1在卵巢发育阶段上调，chfr、ampk、aldh2、dao、glt1d1、hvcn1、psmd9、srsf9和ubc在睾丸发育阶段上调。本研究的结果为中国软鳖的性别决定和分化提供了初步的见解。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.