Performance of a Mycoplasma hyopneumoniae serum ELISA for antibody detection in processing fluids

IF 0.7 4区农林科学 Q3 Agricultural and Biological Sciences

Journal of Swine Health and Production Pub Date : 2022-05-02 DOI:10.54846/jshap/1265

R. Magtoto, B. Armenta-Leyva, P. Dizon-Magtoto, Ting-Yu Cheng, M. Clavijo, C. Johnson, W. Lopez, D. Baum, J. Zimmerman, L. Gimenez-Lirola

引用次数: 0

Abstract

The diagnostic performance of a commercial Mycoplasma hyopneumoniae (MHP) serum enzyme-linked immunosorbent assay (ELISA) was evaluated for MHP antibody detection in processing fluids (n = 494) using samples from three commercial swine farms. Based on historical monitoring, one farm was considered MHP positive and two were considered MHP negative. Samples were tested at a 1:10 dilution and diagnostic sensitivities and specificities estimated for specific ELISA sample-to-positive (S:P) cutoffs. At S:P ≥ 0.40, diagnostic sensitivity and specificity were estimated as 97.6% and 100.0%, respectively. Overall, the results suggest that processing fluids can be used for MHP antibody surveillance in breeding herds.

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猪肺炎支原体血清ELISA检测加工液中抗体的性能

使用来自三个商业养猪场的样本，评估了商业猪肺炎支原体（MHP）血清酶联免疫吸附试验（ELISA）在加工液（n=494）中检测MHP抗体的诊断性能。根据历史监测，一个农场被认为是MHP阳性，两个农场被视为MHP阴性。样品以1:10的稀释度进行测试，并估计特定ELISA样品的诊断敏感性和特异性为阳性（S:P）临界值。当S:P≥0.40时，诊断灵敏度和特异性分别为97.6%和100.0%。总体而言，研究结果表明，加工液可用于繁殖群中MHP抗体的监测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Swine Health and Production 农林科学-兽医学

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>36 weeks

期刊介绍： The Journal of Swine Health & Production (JSHAP) is an open-access and peer-reviewed journal published by the American Association of Swine Veterinarians (AASV) since 1993. The aim of the journal is the timely publication of peer-reviewed papers with a scope that encompasses the many domains of applied swine health and production, including the diagnosis, treatment, management, prevention and eradication of swine diseases, welfare & behavior, nutrition, public health, epidemiology, food safety, biosecurity, pharmaceuticals, antimicrobial use and resistance, reproduction, growth, systems flow, economics, and facility design. The journal provides a platform for researchers, veterinary practitioners, academics, and students to share their work with an international audience. The journal publishes information that contains an applied and practical focus and presents scientific information that is accessible to the busy veterinary practitioner as well as to the research and academic community. Hence, manuscripts with an applied focus are considered for publication, and the journal publishes original research, brief communications, case reports/series, literature reviews, commentaries, diagnostic notes, production tools, and practice tips. All manuscripts submitted to the Journal of Swine Health & Production are peer-reviewed.