Removal of Nickel, Copper, Lead and Cadmium by New Strains of Sphingomonas melonis E8 and Enterobacter hormaechei WW28

Q3 Biochemistry, Genetics and Molecular Biology

Journal of Applied Biotechnology Reports Pub Date : 2020-12-01 DOI:10.30491/JABR.2020.120185

P. Heidari, Samaneh Sanaeizade, Faezeh Mazloomi

{"title":"Removal of Nickel, Copper, Lead and Cadmium by New Strains of Sphingomonas melonis E8 and Enterobacter hormaechei WW28","authors":"P. Heidari, Samaneh Sanaeizade, Faezeh Mazloomi","doi":"10.30491/JABR.2020.120185","DOIUrl":null,"url":null,"abstract":"Introduction: Bioremediation as an eco-friendly technique has a high potential to clean-up the toxicity of heavy metals from contaminated soil and water. In this study, the bioremediation potential of E8 and WW28 strains which had high similarity to Sphingomonas melonis and Enterobacter hormaechei, respectively have been evaluated under contaminated mediums with lead, cadmium, copper, and nickel. Materials and Methods: The growth rate and metal removal percentage of isolated strains were investigated at different ranges of pH 4-8, and temperature (25, 30, 35, and 40 ˚C). Also, the bioremediation potential of isolated strains was studied under a mixture of metals (50 mg/L of each metal). Results: The highest cell mass of strain E8 was observed after 48h at 30 ˚C and pH 5 while strains WW28 showed a high growth rate after 72h at 25 ˚C and pH 5. Strains E8 and WW28 preferred to more uptake nickel and copper than lead and cadmium. In addition, cadmium appears to show the highest toxicity towards the isolated bacteria. Strain E8 as multi metals-resistance strain could remove 78, 62, and 56% of nickel, copper, and cadmium, respectively from polluted mediums at pH 6 after 48h. Conclusions: Overall results revealed that isolated strains as bio-tools have a high potential to be used in the bioremediation process of nickel and multi-metals contaminated sites.","PeriodicalId":14945,"journal":{"name":"Journal of Applied Biotechnology Reports","volume":"7 1","pages":"208-214"},"PeriodicalIF":0.0000,"publicationDate":"2020-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"7","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Biotechnology Reports","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.30491/JABR.2020.120185","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Biochemistry, Genetics and Molecular Biology","Score":null,"Total":0}

引用次数: 7

Abstract

Introduction: Bioremediation as an eco-friendly technique has a high potential to clean-up the toxicity of heavy metals from contaminated soil and water. In this study, the bioremediation potential of E8 and WW28 strains which had high similarity to Sphingomonas melonis and Enterobacter hormaechei, respectively have been evaluated under contaminated mediums with lead, cadmium, copper, and nickel. Materials and Methods: The growth rate and metal removal percentage of isolated strains were investigated at different ranges of pH 4-8, and temperature (25, 30, 35, and 40 ˚C). Also, the bioremediation potential of isolated strains was studied under a mixture of metals (50 mg/L of each metal). Results: The highest cell mass of strain E8 was observed after 48h at 30 ˚C and pH 5 while strains WW28 showed a high growth rate after 72h at 25 ˚C and pH 5. Strains E8 and WW28 preferred to more uptake nickel and copper than lead and cadmium. In addition, cadmium appears to show the highest toxicity towards the isolated bacteria. Strain E8 as multi metals-resistance strain could remove 78, 62, and 56% of nickel, copper, and cadmium, respectively from polluted mediums at pH 6 after 48h. Conclusions: Overall results revealed that isolated strains as bio-tools have a high potential to be used in the bioremediation process of nickel and multi-metals contaminated sites.

查看原文本刊更多论文

甜瓜鞘氨醇单胞菌E8和贺氏肠杆菌WW28对镍、铜、铅和镉的去除作用

简介：生物修复作为一种环保技术，在清除污染土壤和水中重金属的毒性方面具有很高的潜力。在本研究中，在铅、镉、铜和镍污染的介质中，分别评估了E8和WW28菌株的生物修复潜力，这两个菌株分别与甜瓜鞘氨醇单胞菌和贺氏肠杆菌具有高度相似性。材料和方法：在pH 4-8和温度（25、30、35和40˚C）的不同范围内，研究分离菌株的生长速率和金属去除率。此外，在金属混合物（每种金属50mg/L）下研究分离菌株的生物修复潜力。结果：菌株E8在30˚C和pH 5下48小时后细胞质量最高，而菌株WW28在25˚C、pH 5下72小时后细胞生长率较高。菌株E8和WW28优选比铅和镉吸收更多的镍和铜。此外，镉似乎对分离的细菌显示出最高的毒性。菌株E8作为耐多金属菌株，在pH6的污染介质中，48h后可分别去除78%、62%和56%的镍、铜和镉。结论：总体结果表明，分离菌株作为生物工具在镍和多种金属污染场地的生物修复过程中具有很高的应用潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Journal of Applied Biotechnology Reports Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

1.90

自引率

0.00%

发文量

期刊介绍： The Journal of Applied Biotechnology Reports (JABR) publishes papers describing experimental work relating to all fundamental issues of biotechnology including: Cell Biology, Genetics, Microbiology, Immunology, Molecular Biology, Biochemistry, Embryology, Immunogenetics, Cell and Tissue Culture, Molecular Ecology, Genetic Engineering and Biological Engineering, Bioremediation and Biodegradation, Bioinformatics, Biotechnology Regulations, Pharmacogenomics, Gene Therapy, Plant, Animal, Microbial and Environmental Biotechnology, Nanobiotechnology, Medical Biotechnology, Biosafety, Biosecurity, Bioenergy, Biomass, Biomaterials and Biobased Chemicals and Enzymes. Journal of Applied Biotechnology Reports promotes a special emphasis on: -Improvement methods in biotechnology -Optimization process for high production in fermentor systems -Protein and enzyme engineering -Antibody engineering and monoclonal antibody -Molecular farming -Bioremediation -Immobilizing methods -biocatalysis