Bacteria associated with leaf-cutter ants drive natural antibiotic resistance in soil bacteria

IF 1 4区环境科学与生态学 Q4 ECOLOGY

Journal of Tropical Ecology Pub Date : 2022-07-18 DOI:10.1017/s0266467422000323

Sophia Simon, Kelsey Chai, Matthew Drescher, J. Chaves-Campos

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引用次数: 1

Abstract

Actinobacteria that live mutualistically with leaf-cutter ants secrete antibiotics that may induce antibiotic resistance in nearby soil bacteria. We tested for the first time whether soil bacteria near and inside Atta cephalotes nests in Costa Rica show higher levels of antibiotic resistance than bacteria collected farther away. We collected soil samples 0 m to 50 m away from ant nests and grew bacteria from them on agar with paper discs treated with antibiotics of common veterinary use. As a proxy for antibiotic resistance, we measured the distance from the edge of each disc to the closest bacterial colonies. In general, resistance to oxytetracycline increased with proximity to leaf-cutter ant nests. Antibiotic resistance to oxytetracycline was also higher in samples collected inside the nest than in samples from the nest mound; not all antibiotics demonstrated the same trend. A preliminary exploratory morphological analysis suggests bacterial communities between 0 m and 50 m from ant nests were similar in diversity and abundance, indicating the pattern of antibiotic resistance described above may not be caused by differences in community composition. We conclude that actinobacteria living mutualistically with A. cephalotes drive natural antibiotic resistance to tetracycline in proximal bacterial communities.

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与切叶蚁相关的细菌驱动土壤细菌的天然抗生素耐药性

与切叶蚁共生的放线菌分泌抗生素，可诱导附近土壤细菌产生耐药性。我们首次测试了哥斯达黎加Atta cephalalotes巢穴附近和内部的土壤细菌是否比远处收集的细菌显示出更高的抗生素耐药性。我们收集了距离蚁巢0米至50米的土壤样本，并在琼脂上用普通兽医使用的抗生素处理过的纸盘培养细菌。作为抗生素耐药性的代表，我们测量了从每个圆盘的边缘到最近的细菌菌落的距离。一般来说，对土霉素的耐药性随着靠近切叶蚁巢而增加。巢内采集的样本对土霉素的耐药性也高于巢丘采集的样本;并非所有抗生素都表现出同样的趋势。初步的探索性形态学分析表明，距离蚁巢0 m至50 m的细菌群落在多样性和丰度上相似，这表明上述抗生素耐药性模式可能不是由群落组成差异引起的。我们得出结论，放线菌与A.头孢菌共生驱动近端细菌群落对四环素的天然耐药性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Tropical Ecology 环境科学-生态学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

18-36 weeks

期刊介绍： Journal of Tropical Ecology aims to address topics of general relevance and significance to tropical ecology. This includes sub-disciplines of ecology, such as conservation biology, evolutionary ecology, marine ecology, microbial ecology, molecular ecology, quantitative ecology, etc. Studies in the field of tropical medicine, specifically where it involves ecological surroundings (e.g., zoonotic or vector-borne disease ecology), are also suitable. We also welcome methods papers, provided that the techniques are well-described and are of broad general utility. Please keep in mind that studies focused on specific geographic regions or on particular taxa will be better suited to more specialist journals. In order to help the editors make their decision, in your cover letter please address the specific hypothesis your study addresses, and how the results will interest the broad field of tropical ecology. While we will consider purely descriptive studies of outstanding general interest, the case for them should be made in the cover letter.