Real-time scanning electron microscopy of unfixed tissue in the solution using a deformable and electron-transmissive film

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2022-06-17 DOI:10.1093/jmicro/dfac030

Seine A. Shintani, S. Yamaguchi, H. Takadama

引用次数: 0

Abstract

Abstract It is difficult to use scanning electron microscopy to observe the structure and movement of biological tissue immersed in the solution. To enable such observations, we created a highly deformable and electron-transmissive polyimide film that can withstand the pressure difference between the high-vacuum electron column and the atmospheric-pressure sample chamber. With this film, we used scanning electron microscopy to measure the intrinsic fine structure and movement of the contractile fibers of excised mouse heart immersed in physiological solutions. Our measurements revealed that the excised heart is a dynamic tissue that undergoes relaxation oscillation based on a three-dimensional force balance.

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使用可变形和电子透射膜对溶液中未固定组织进行实时扫描电子显微镜检查

摘要用扫描电子显微镜很难观察浸泡在溶液中的生物组织的结构和运动。为了进行这样的观察，我们制作了一种高度可变形和电子透射的聚酰亚胺薄膜，它可以承受高真空电子柱和大气压样品室之间的压力差。利用这种薄膜，我们使用扫描电子显微镜测量了浸泡在生理溶液中的离体小鼠心脏收缩纤维的内在精细结构和运动。我们的测量结果表明，切除的心脏是一个动态组织，基于三维力平衡进行松弛振荡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.