Effect of activated charcoal and ascorbic acid on in vitro morphogenesis and o-dihydroxyphenols content in Paphiopedilum insigne

IF 1 Q3 HORTICULTURE

Horticultural Science Pub Date : 2022-01-05 DOI:10.17221/68/2020-hortsci

Monika Poniewozik, M. Parzymies, P. Szot

引用次数: 0

Abstract

Phenolic compounds limit micropropagation of many orchids in vitro. The aim of the study was to estimate the effect of activated charcoal (AC);1, 2 or 4 g/L) or ascorbic acid (AA; 10, 20 or 30 mg/L) added to the half strength MS medium on the growth and o-dihydroxyphenols content in Paphiopedilum insigne in vitro. A positive effect of AC on the shoot and root formation has been found. The highest multiplication rate (5.6 shoots/explant) and rooting frequency were obtained on medium containing 2 g/L of AC. However, AC reduced the leaf number as compared to the control. The lowest content of o-dihydroxyphenols was marked in Paphiopedilum insigne leaves when the shoots were grown on medium with 10 mg/L AA, followed by AC at 1 or 2 g/L.

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活性炭和抗坏血酸对罂粟叶体外形态发生及邻二羟基酚含量的影响

酚类化合物限制了许多兰花在体外的微繁殖。该研究的目的是评估活性炭(AC)(1、2或4 g/L)或抗坏血酸(AA;在半强度MS培养基中添加10、20、30 mg/L)对离体罂粟叶生长及邻二羟基酚含量的影响。研究发现，AC对茎和根的形成有积极的影响。在添加2 g/L AC的培养基上，植株的增殖率最高(5.6个芽/外植体)，生根频率最高，但与对照相比，AC减少了叶片数。在AA浓度为10 mg/L的培养基上，邻二羟基酚含量最低，AC浓度为1、2 g/L的培养基次之。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Horticultural Science Horticulture-园艺

CiteScore

2.20

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： The journal publishes results of basic and applied research from all areas of horticulture, fruit-growing, vegetable-growing, wine-making and viticulture, floriculture, ornamental gardening, garden and landscape architecture, concerning plants that are grown under the conditions of European temperate zone, or field plants that are considered as horticultural cultures. Original scientific papers, short communications and review articles are published in the journal. Papers are published in English (British spelling).