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Abstract

Cover: In Kasprick et al. (https://doi.org/10.1002/cpph.55), the image shows analysis of skin sections. (A) Determination of titer from rabbits immunized with mCOL7^vWFA2. Cryosections of normal mouse skin were incubated with serum diluted 1:4,000 1:8,000, 1:16,000, and 1:32,000 and then stained with FITC-AffiniPure donkey anti-rabbit IgG as described in Basic Protocol 3. As the serum specifically binds to the dermal-epidermal junction of the skin, a typical green line is visible (arrows). The line visible at the lowest tested concentration determines the titer (here, 1:8,000). (B) Analysis of H&E staining. Typical ear sections 12 days after first IgG injection in antibody transfer–induced EBA. Upper left picture shows a mild inflammation with less thickening and only a few inflammatory cells. Other pictures show typical thickening of the epidermal layers and massive inflammation. Arrows indicate split formation at dermal-epidermal junction; ^**indicates cartilage of the ear.