Identification of model systems expressing circFAT3

Abstract

Recent RNA-seq studies have identified thousands of potential circular RNAs (circRNAs) in a variety of organisms and tissues. While experimental studies have begun to elucidate the biological roles of some circRNAs, the functions of the vast majority are not yet understood. As a first step to determining circRNA function, molecular techniques must be used to verify their expression in suitable model systems. In this study we use PCR and Sanger sequencing to identify model systems in three different organisms (human, chicken, and zebrafish) that each express circFAT3, a 3-kb circular RNA derived from the gene FAT3. We further characterize the expression levels of circFAT3 and its host mRNA during chicken retinal development. Our results suggest that circFAT3 is evolutionarily conserved and shows a dynamic expression pattern that is independent of its linear host mRNA.