In vitro assessment of anti-diabetic potential of 4 kinds of dark tea (Camellia sinensis L.) protein hydrolysates

IF 1.2 4区生物学 Q2 Agricultural and Biological Sciences

Journal of Applied Botany and Food Quality-Angewandte Botanik Pub Date : 2019-03-04 DOI:10.5073/JABFQ.2019.092.008

Keying Su, Xinliang Mao, Li Ai, Xuewu Zhang

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引用次数: 8

Abstract

The contributions of four kinds of dark tea (Camellia sinensis L.) proteins and their hydrolysates to hypoglycemic activity were investigated in vitro. Four kinds of water-extracted dark tea proteins were hydrolyzed with trypsin and Alcalase, respectively. The complete proteins had α-amylase inhibitory activity with half maximal inhibitory concentration (IC50) values ranging from 1.27 to 2.78 mg/mL. Most of the dark tea proteins and hydrolysates significantly inhibited α-glucosidase and dipeptidyl peptidase (DPP-IV), with IC50 values in the range of 0.0103-1.3114 mg/mL and 0.1000-1.3364 mg/mL, respectively. In general, Heimaojian (HMJ) and Qianliang (QL) hydrolysates displayed high α-glucosidase inhibitory activity, while HMJ, Fuzhuan (FZ), and Heizhuan (HZ) hydrolysates exhibited a strong ability to inhibit DPP-IV. This study demonstrates the potential of dark tea proteins and their hydrolysates as a source of functional food and medicine for the control of type 2 diabetes.

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4种黑茶蛋白水解物抗糖尿病作用的体外评价

研究了四种黑茶蛋白及其水解产物对体外降血糖的作用。用胰蛋白酶和Alcalase分别对4种水提黑茶蛋白进行水解。完整蛋白具有α-淀粉酶抑制活性，半数最大抑制浓度(IC50)为1.27 ~ 2.78 mg/mL。大部分黑茶蛋白和水解产物对α-葡萄糖苷酶和二肽基肽酶(DPP-IV)的抑制作用显著，IC50值分别为0.0103 ~ 1.3114 mg/mL和0.1000 ~ 1.3364 mg/mL。总体而言，黑毛尖(HMJ)和钱亮(QL)水解产物具有较高的α-葡萄糖苷酶抑制活性，而HMJ、扶直(FZ)和黑直(HZ)水解产物具有较强的抑制DPP-IV的能力。这项研究证明了黑茶蛋白及其水解物作为控制2型糖尿病的功能性食品和药物来源的潜力。

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来源期刊

Journal of Applied Botany and Food Quality-Angewandte Botanik 生物-植物科学

CiteScore

2.50

自引率

0.00%

发文量

审稿时长

20 weeks

期刊介绍： The Journal of Applied Botany and Food Quality is the Open Access journal of the German Society for Quality Research on Plant Foods and the Section Applied Botany of the German Botanical Society. It provides a platform for scientists to disseminate recent results of applied plant research in plant physiology and plant ecology, plant biotechnology, plant breeding and cultivation, phytomedicine, plant nutrition, plant stress and resistance, plant microbiology, plant analysis (including -omics techniques), and plant food chemistry. The articles have a clear focus on botanical and plant quality aspects and contain new and innovative information based on state-of-the-art methodologies.