Roles of Long Non-coding Ribonucleic Acid X Inactive Specific Transcript/microRNA-29a/phosphatase and Tensin Homolog Deleted on Chromosome Ten Pathway in Osteogenic Differentiation of Bone Marrow Mesenchymal Stem Cells and Postmenopausal Osteoporosis

Pub Date : 2022-09-01 DOI:10.31901/24566330.2022/22.03.803

Jian Liu

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Abstract

The researchers aimed to inquire into the roles of long non-coding ribonucleic acid X inactive specific transcript/microRNA-29a/phosphatase and tensin homolog deleted on chromosome ten (lncRNA-XIST/miR-29a/ PTEN) pathway in the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) and postmenopausal osteoporosis (PMOP). In the miR-29a + Lenti-NC group, ALP activity, concentration of alizarin red, and mRNA and protein expressions of Runx2, OPN and OCN significantly increased, whereas the mRNA and protein expressions of LPL, AP-2 and leptin decreased (P<0.05). In contrast with the miR-29a + Lenti-NC group, miR-29a + Lenti-XIST and miR-29a + Lenti-PTEN groups had decreased ALP activity, concentration of alizarin red, and mRNA and protein expressions of Runx2, OPN and OCN, but increased mRNA and protein expressions of LPL, AP-2 and leptin (P<0.05). BMSCs have incremental expressions of lncRNA-XIST and PTEN and a declined expression of miR-29a. LncRNAXIST suppresses the osteogenic differentiation of BMSCs by feat of the miR-29a/PTEN pathway.

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长非编码核糖核酸X无活性特异性转录物/microRNA-29a/磷酸酶和10号染色体通路上张力素同源物缺失在骨髓间充质干细胞成骨分化和绝经后骨质疏松中的作用

研究人员旨在探讨长链非编码核糖核酸X失活特异性转录物/microRNA-29a/磷酸酶和10号染色体上缺失的紧张素同源物(lncRNA-XIST/miR-29a/ PTEN)通路在骨髓间质干细胞(BMSCs)成骨分化和绝经后骨质疏松症(PMOP)中的作用。miR-29a + lentic - nc组ALP活性、茜素红浓度及Runx2、OPN、OCN mRNA和蛋白表达量显著升高，LPL、AP-2、leptin mRNA和蛋白表达量显著降低(P<0.05)。与miR-29a + lentii - nc组相比，miR-29a + lentii - xist、miR-29a + lentii - pten组ALP活性、茜素红浓度、Runx2、OPN、OCN mRNA及蛋白表达均降低，LPL、AP-2、leptin mRNA及蛋白表达升高(P<0.05)。骨髓间质干细胞lncRNA-XIST和PTEN的表达增加，miR-29a的表达下降。LncRNAXIST通过miR-29a/PTEN途径抑制BMSCs的成骨分化。

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